Protein Info for GFF7228 in Variovorax sp. SCN45

Annotation: Ribonucleotide reductase of class II (coenzyme B12-dependent) (EC 1.17.4.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00317: Ribonuc_red_lgN
  • amino acids 22 to 87 (66 residues), 36.1 bits, E=5.7e-13
• TIGR02504: ribonucleoside-diphosphate reductase, adenosylcobalamin-dependent
  • amino acids 35 to 593 (559 residues), 558.1 bits, E=1.3e-171
• PF02867: Ribonuc_red_lgC
  • amino acids 93 to 585 (493 residues), 392.5 bits, E=3.1e-121

Predicted SEED Role

"Ribonucleotide reductase of class II (coenzyme B12-dependent) (EC 1.17.4.1)" in subsystem Ribonucleotide reduction (EC 1.17.4.1)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (43/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (24/26 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (17/18 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• superpathway of purine nucleotide salvage (12/14 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (12/14 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis I (6/6 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• superpathway of adenosine nucleotides de novo biosynthesis I (5/5 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis II (7/8 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• superpathway of adenosine nucleotides de novo biosynthesis II (6/7 steps found)
• adenosine deoxyribonucleotides de novo biosynthesis I (2/2 steps found)
• guanosine deoxyribonucleotides de novo biosynthesis I (2/2 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• adenosine deoxyribonucleotides de novo biosynthesis II (3/4 steps found)
• guanosine deoxyribonucleotides de novo biosynthesis II (3/4 steps found)

KEGG Metabolic Maps

• Glutathione metabolism
• Purine metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.17.4.1

Use Curated BLAST to search for 1.17.4.1

Protein Sequence (616 amino acids)

>GFF7228 Ribonucleotide reductase of class II (coenzyme B12-dependent) (EC 1.17.4.1) (Variovorax sp. SCN45)
MAMTRFTVDSPRLPELPVQPICRDVLMEKYAQAGEHSIDDIHRRVARALAQAEKPGEREA
WEARFLQALRDGFVPAGRIQSAAGTALTATLINCFVQPVGDSITGDDEGYPGIYVALAEA
AETMRMGGGVGYDFSRIRPHGAWVAGTRSSASGPVPFMRVFDCSCRTVESAGARRGAQMG
VLRCDHPDIEAFMGAKDDGELSNFNLSVGLTDAFMLAVIDDREIELVHCAEPGPRIGSAQ
EHRRDDGLWVYARRPARALWDRIMRSAYAHGEPGVLFLDRINADNNLSSCETLAATNPCG
EQPLPPYGSCCLGSIDLTRFVKDPFDPGARFDEKNFSRLAGLAVRMLDNVLDITAWPLER
QGREALLKRRIGLGFTGLGDALVMLNLRYDAQPGRAAARRIARLLRDAAYGASCALARER
GAFPLFDAGLYLGHGTFASRLPEPMKTRIRAYGMRNSHLLSVAPTGTISLAFSDNASNGI
EPAFAWSFVRRKRLPEGGTREYEVEDHALRLYRQRRGERAALGPAFVTALEMSPSAHVEM
VAAVAPYVDAAISKTVNVPSDCAYEDFKELYMQAWQSGLKGLAAYRPNPLRGAVLKAPAR
PACSGRAAPVAPVAPA