Protein Info for GFF7214 in Variovorax sp. SCN45

Annotation: Peptidase M20D, amidohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 436 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details TIGR01891: amidohydrolase" amino acids 43 to 393 (351 residues), 282.4 bits, see alignment E=2.8e-88 PF01546: Peptidase_M20" amino acids 99 to 388 (290 residues), 100.6 bits, see alignment E=1.2e-32 PF07687: M20_dimer" amino acids 220 to 314 (95 residues), 48.6 bits, see alignment E=7.1e-17

Best Hits

KEGG orthology group: K01451, hippurate hydrolase [EC: 3.5.1.32] (inferred from 66% identity to rpi:Rpic_2778)

Predicted SEED Role

"N-acetyl-L,L-diaminopimelate deacetylase (EC 3.5.1.47)" in subsystem Lysine Biosynthesis DAP Pathway (EC 3.5.1.47)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.32, 3.5.1.47

Use Curated BLAST to search for 3.5.1.32 or 3.5.1.47

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (436 amino acids)

>GFF7214 Peptidase M20D, amidohydrolase (Variovorax sp. SCN45)
MHLPAFRQLSLLAMCAIAPAAFAQQPTARIDALVDGMYPSLETIYKDIHSNPELSFKETR
TAAKLAAEMRDLGFEVTEKVGRTGIVAIYKNGPGPTVLVRTELDALPMEEKTGLPYASKV
KAEWNGRETFVAHSCGHDIHMASWVGTARTLLALKDQWKGTLMFIGQPAEEAGGGAKAML
ADGLFKRFPKPDFAFALHTSPSPYGFVGYRVGAITSASDGLEITFKGRGGHGSAPDKTID
PIAIAAHFVTDVQTVVSREKDPAEFGVVTIGAIQGGTAGNIIPDSVVLRGTVRSYKPEVR
EKLLAGVRRTAKAAAAMAGAPEPVVEFGDGGAAVINDEAVVQRTVAALKSKLDAARVVQV
PPITASEDFSEYVNAGVPSMFFFVGVSDPKEVAESLKPGGKPLPFNHSPFFAPVPEPSIK
MGVQAMTTAVLSAMNK