Protein Info for GFF7213 in Variovorax sp. SCN45

Annotation: DNA polymerase II (EC 2.7.7.7)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 804 PF21474: DNApolII_N" amino acids 22 to 57 (36 residues), 66.2 bits, see alignment (E = 2.6e-22) PF03104: DNA_pol_B_exo1" amino acids 93 to 315 (223 residues), 39.4 bits, see alignment E=6.5e-14 PF00136: DNA_pol_B" amino acids 397 to 788 (392 residues), 122.3 bits, see alignment E=3.8e-39

Best Hits

Swiss-Prot: 56% identical to DPO2_ECOLI: DNA polymerase II (polB) from Escherichia coli (strain K12)

KEGG orthology group: K02336, DNA polymerase II [EC: 2.7.7.7] (inferred from 87% identity to vap:Vapar_6015)

MetaCyc: 56% identical to DNA polymerase II (Escherichia coli K-12 substr. MG1655)
3.1.11.-; DNA-directed DNA polymerase. [EC: 2.7.7.7]

Predicted SEED Role

"DNA polymerase II (EC 2.7.7.7)" in subsystem DNA-replication or DNA Repair Base Excision (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (804 amino acids)

>GFF7213 DNA polymerase II (EC 2.7.7.7) (Variovorax sp. SCN45)
VPAPAPTLSPILPAPVAPSLQGFILTRHWRDASSGTEIEYWLATDEGPRKAVLRSQTSVA
FVESRHRPALEAQLAAMPGMQVRELELKTFNCQPVVGVYAKHYRQLGRLTRALQPQGVPV
LEADVRPHDRYLMERFITAGVTVEEGRADGATIVDCRLKPAPEFRPVLKMVSLDIETSQD
EALYSIALDGLQDRVVFMLGEAPPGPGEPMDFSLVYCPTRKAMVESLNDWFERNDPDVVI
GWNVIQFDLRVLQKTANDCGMQLLLGRERRPIEWRTHPGKQGYLFAPTPGRVIIDGIDAL
KAAMWSFPSFSLETVSQALLGEGKAIGDEYDKMAEIERRYQEDKPALALYNIRDCELVLR
IFDKAKLLQFVMERAQTTGLQADHFGGSIAAFSHHYLPRMHRLGYVAPNVGEIASKAFPG
GYVMDSKPGFYDSVVVLDYKSLYPSIIRTFLVDPVGLVEGTHAGDPAMVVKGPQGTVFSR
ERHCLPEIVTTLWRARDEAKRVGNEPLSQALKLLMNSFAGVLGAADCRFFNPKLVSAVTL
RGHEMMKLTREFVQSRGYEVIYGDTDSIFVWLKRTHTNEEAHAVAANLVRDINDWWTGSL
RDEQGLDNFLEIEFDTHYRKFFMPTIRGSDVGSKKRYAGLSVDAKGKEEMVYRGLEMARS
DWTPLARQFQEGLLSRIFQGEPYKEFVSDYAQSTLAGEKDDLLIYRKRLRHRLDAYLVNV
PPQVRAARIADEYNARIGRPMQYQSGGWIRYVMTRNGPEPLETRHSRIDYEHYLSKQLQP
IADAILQPVGESFTALTTAQQHLF