Protein Info for GFF72 in Xanthobacter sp. DMC5

Annotation: Tol-Pal system protein TolQ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 268 signal peptide" amino acids 1 to 35 (35 residues), see Phobius details transmembrane" amino acids 58 to 82 (25 residues), see Phobius details amino acids 170 to 193 (24 residues), see Phobius details amino acids 213 to 235 (23 residues), see Phobius details TIGR02796: protein TolQ" amino acids 50 to 262 (213 residues), 283.3 bits, see alignment E=6.8e-89 PF01618: MotA_ExbB" amino acids 145 to 250 (106 residues), 122.1 bits, see alignment E=6.3e-40

Best Hits

Swiss-Prot: 42% identical to TOLQ_ECOLI: Tol-Pal system protein TolQ (tolQ) from Escherichia coli (strain K12)

KEGG orthology group: K03562, biopolymer transport protein TolQ (inferred from 91% identity to xau:Xaut_3059)

Predicted SEED Role

"MotA/TolQ/ExbB proton channel family protein" in subsystem Ton and Tol transport systems

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (268 amino acids)

>GFF72 Tol-Pal system protein TolQ (Xanthobacter sp. DMC5)
MIDVTSRTGTVSRLTPAIATAVGLLLLGTLPALAQDPIGSAVPPPAADFSFLGLFLQAHI
VVKLVMIGLIVSSIWVWAIIINKWMLFSRNKREMDRFEQVFWSGQSLEDLYRTLAGRSNH
GMAAIFVAAMREWKRTYEGGARSLAGLNQRLDKVMNVTIAREVERLENSLLVLATVGSAG
PFVGLFGTVWGIMTSFQSIAASKNTSLAVVAPGIAEALFATAMGLIAAIPATIFYNKFIS
QVGRQAQRLEGFADEFSAILSRQIDERG