Protein Info for GFF716 in Pseudomonas sp. DMC3

Annotation: putative copper-importing P-type ATPase A

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 816 transmembrane" amino acids 179 to 199 (21 residues), see Phobius details amino acids 213 to 234 (22 residues), see Phobius details amino acids 246 to 268 (23 residues), see Phobius details amino acids 274 to 292 (19 residues), see Phobius details amino acids 427 to 448 (22 residues), see Phobius details amino acids 454 to 478 (25 residues), see Phobius details amino acids 753 to 770 (18 residues), see Phobius details amino acids 776 to 794 (19 residues), see Phobius details PF12156: ATPase-cat_bd" amino acids 7 to 89 (83 residues), 100.6 bits, see alignment E=2.1e-32 PF00403: HMA" amino acids 96 to 156 (61 residues), 34.5 bits, see alignment 6.4e-12 TIGR01511: copper-translocating P-type ATPase" amino acids 231 to 796 (566 residues), 470.7 bits, see alignment E=1.5e-144 TIGR01525: heavy metal translocating P-type ATPase" amino acids 248 to 796 (549 residues), 515.3 bits, see alignment E=5e-158 TIGR01512: cadmium-translocating P-type ATPase" amino acids 276 to 797 (522 residues), 371.2 bits, see alignment E=1.7e-114 TIGR01494: HAD ATPase, P-type, family IC" amino acids 284 to 777 (494 residues), 224.8 bits, see alignment E=3.2e-70 PF00122: E1-E2_ATPase" amino acids 310 to 487 (178 residues), 171.1 bits, see alignment E=5.6e-54 PF00702: Hydrolase" amino acids 504 to 708 (205 residues), 101.1 bits, see alignment E=3.2e-32 PF12710: HAD" amino acids 593 to 704 (112 residues), 30.1 bits, see alignment E=1.9e-10 PF08282: Hydrolase_3" amino acids 679 to 739 (61 residues), 37.9 bits, see alignment 5.3e-13

Best Hits

KEGG orthology group: K01533, Cu2+-exporting ATPase [EC: 3.6.3.4] (inferred from 94% identity to pfo:Pfl01_1816)

Predicted SEED Role

"Type cbb3 cytochrome oxidase biogenesis protein CcoI; Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.4)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.4

Use Curated BLAST to search for 3.6.3.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (816 amino acids)

>GFF716 putative copper-importing P-type ATPase A (Pseudomonas sp. DMC3)
MTSPTPCYHCALPVPSGSRFTAVVLGQAREFCCPGCQAVAEAIVAGGLESYYQHRSEASA
NPEALPVQLTDELALYDRADVQQPFVRHEGDLAETTLLMEGISCAACGWLIEKHLRTLPA
VAEARLNLSNHRLHVRWADAQLPLSQILAELRHIGYAAHPYQSDRASEQLASENRLALRQ
LGVAGLLWFQAMMATMATWPEFNIDLSPELHTILRWVALFLTTPIVFYSCAPFFKGAMRD
LRTRHLTMDVSVSLAIGSAYIAGIWTSITGVGELYFDAVGMFALFLLAGRYLERRARERT
AAATAQLVNLLPASCLRLDSDGQSERILLSELRVGERILVQPGSILPADGKIIDGQSSID
ESLLTGEYLPQPRTLGDAVTAGTLNVEGALTVEVQALGQDTRLSAIVRLLDRAQAEKPRL
AEIADRAAQWFLLLSLIAAAVIGLLWWQLDSSRAFWIVLAMLVATCPCALSLATPTALTA
ATGTLHKLGLLLTRGHVLEGLNQIDTVIFDKTGTLTEGRLVLRSIRPLGALDSEQCLSLA
AALENRSEHPIARAFGRAPLAAEDVHSTPGLGLEGVVGEQRLRIGQAEFACALSGAALPS
MPDEAGQWLLLADTHGPLAWLVLDDRLRDDAPALLAACKARGWRTLLLSGDSSPMVASVA
AELGIDEARGGLRPDDKLQVLQQLHKEGRKVLMLGDGVNDVPVLAAADISVAMGSATDLA
KTSADAVLLSNRLDALVQAFTLARRTRRVIIENLLWAALYNGLMLPFAALGWITPVWAAV
GMSISSLTVVLNALRLTRLPSAPAAGSPTQTRPLPA