Protein Info for GFF7088 in Variovorax sp. SCN45

Annotation: Benzoate 1,2-dioxygenase alpha subunit (EC 1.14.12.10)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 455 PF00355: Rieske" amino acids 44 to 127 (84 residues), 67.1 bits, see alignment E=1e-22 PF00848: Ring_hydroxyl_A" amino acids 196 to 435 (240 residues), 64.8 bits, see alignment E=1.1e-21

Best Hits

KEGG orthology group: K00470, [EC: 1.14.1.-] (inferred from 83% identity to bbr:BB0728)

Predicted SEED Role

"3-phenylpropionate dioxygenase alpha subunit (EC 1.14.1.-)" (EC 1.14.1.-)

Isozymes

Compare fitness of predicted isozymes for: 1.14.1.-

Use Curated BLAST to search for 1.14.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (455 amino acids)

>GFF7088 Benzoate 1,2-dioxygenase alpha subunit (EC 1.14.12.10) (Variovorax sp. SCN45)
MIPIKTESRYNAMVKLREGKISREIFSDKEIFQEELEKVFTRAWLFVGHESQIPNPGDYF
TSRMGAESVILARDKKKKVHVFLNSCRHRGMKVCQYDHGNTQLFTCPYHSWSYTTEGKLF
GVPQFKQLYEGCMEKEEWSLIEPPKMETYKGTVWASWDKDAPDLLTYLGDAKAHLDLALD
CRDGREGGSEVLVGVHKWIIPCNWKFAAENFLGDTYHNVSHRSVDLIGIGPSAEAGVKGR
RDNEMDKAKHVWANFPAGHGVHSAIMPEDWEFLNTYQNNPIVAEYFREAHAKRKERMGAE
KSRLIPFVGTIYPNVSFHGNQPRNLCVWHPHGPESTEAWRFFLVDADAPQEVKDFLRTYY
MRYSGPAGMTEQDDMENWNYATAGSRGVIARRYPFNYTQSLGKVEKDAPVPGNVSLQVSE
ENPRQFYRRWRDYMDGADWDTLLGRKDTQPASFVE