Protein Info for GFF7064 in Variovorax sp. SCN45

Annotation: Glutamate--cysteine ligase (EC 6.3.2.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 501 transmembrane" amino acids 156 to 175 (20 residues), see Phobius details TIGR01434: glutamate--cysteine ligase" amino acids 2 to 480 (479 residues), 487.6 bits, see alignment E=2e-150 PF04262: Glu_cys_ligase" amino acids 2 to 342 (341 residues), 452.2 bits, see alignment E=1.1e-139 PF04107: GCS2" amino acids 32 to 168 (137 residues), 23.1 bits, see alignment E=4.4e-09

Best Hits

KEGG orthology group: K01919, glutamate--cysteine ligase [EC: 6.3.2.2] (inferred from 94% identity to vpe:Varpa_4191)

Predicted SEED Role

"Glutamate--cysteine ligase (EC 6.3.2.2)" in subsystem Glutathione: Biosynthesis and gamma-glutamyl cycle (EC 6.3.2.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.3.2.2

Use Curated BLAST to search for 6.3.2.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (501 amino acids)

>GFF7064 Glutamate--cysteine ligase (EC 6.3.2.2) (Variovorax sp. SCN45)
MRRGIEKESLRALPDGKLALTPHPIALGSALTHPHITTDFSESQLELITGVHGGVESALE
ELTRVHQFTYRVLDGLGDERLWVSSMPCGLPTDETIPIGRYGSSNVGRAKSVYRMGLSHR
YGRRMQTISGIHYNWSLPDVSSEQYFALIRNFRRHAFLLLYLFGASPALCSSFVAGRPHE
LQPLGDGSMFMPHGTSLRMGRLGYQSDAQASLAVSYNSLEGYGASLQDALTRPWPAYESI
GIRNLGGDYNQLGTSLLQIENEFYGTIRPKRVINPGERPLHALRERGVEYVEVRLMDLDP
FETVGINAQTMRFIDVFLLHCLLSDSPDDTPQEIADLARNQHLTAARGREPGLRLARGGS
EVTLADWGVELAEQCLPIAAALDAAHGGGTQHADAVRAALAALQDPDSLPSARVLAAIEQ
QHGNSFVGFVRAQSEQTRAALLALPFSAEQQAAFERMTAESIQEQKRIEAADTMPFEIYR
EQYVSPARLGMTRGRVAALAA