Protein Info for PS417_03545 in Pseudomonas simiae WCS417

Annotation: type III secretion protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 273 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details transmembrane" amino acids 219 to 242 (24 residues), see Phobius details TIGR02544: type III secretion apparatus lipoprotein, YscJ/HrcJ family" amino acids 7 to 198 (192 residues), 201.2 bits, see alignment E=6.7e-64 PF01514: YscJ_FliF" amino acids 23 to 181 (159 residues), 83.9 bits, see alignment E=6.5e-28

Best Hits

KEGG orthology group: K03222, type III secretion protein SctJ (inferred from 75% identity to pfs:PFLU0726)

Predicted SEED Role

"Type III secretion bridge between inner and outermembrane lipoprotein (YscJ,HrcJ,EscJ, PscJ)" in subsystem Type III secretion systems, extended

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UCH6 at UniProt or InterPro

Protein Sequence (273 amino acids)

>PS417_03545 type III secretion protein (Pseudomonas simiae WCS417)
MNHLLPFVLVICLSLLLQACSERVELHRQLTEQEANEVVAELADKHIRATKVPAKDGVIV
VVNATEIARAVRTLEAVGLPRAARTTLGNTFRKEGVISTPLEERARYIYALSQELETTLS
NIDGVIVARVHVVLPERVAPGEPVQPASASVFIKHDPRLDPDSIRARVRRMVASSLPGMA
TAVDNSQKLTVVFVPATAYLEQQRLAYFGPFLVPEDDLGFWRICVTVSLLAVVFMVSGGM
FWQKRKQRVMPTPDVRAQTHEVSSSMTAHSSNE