Protein Info for GFF6925 in Variovorax sp. SCN45

Annotation: Acetyl-CoA synthetase (EC 6.2.1.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02188: acetate--CoA ligase
  • amino acids 28 to 656 (629 residues), 929.5 bits, E=4.2e-284
• PF16177: ACAS_N
  • amino acids 31 to 86 (56 residues), 64.4 bits, 9.8e-22
• PF00501: AMP-binding
  • amino acids 90 to 478 (389 residues), 315.9 bits, E=5.2e-98
• PF13193: AMP-binding_C
  • amino acids 541 to 623 (83 residues), 91 bits, E=1.1e-29

Best Hits

Swiss-Prot: 72% identical to ACSA_BURL3: Acetyl-coenzyme A synthetase (acsA) from Burkholderia lata (strain ATCC 17760 / DSM 23089 / LMG 22485 / NCIMB 9086 / R18194 / 383)

KEGG orthology group: K01895, acetyl-CoA synthetase [EC: 6.2.1.1] (inferred from 79% identity to vap:Vapar_5367)

MetaCyc: 71% identical to acetyl-CoA synthetase (Cupriavidus necator)
Propionate--CoA ligase. [EC: 6.2.1.17]; Acetate--CoA ligase. [EC: 6.2.1.17, 6.2.1.1]

Predicted SEED Role

"Acetyl-coenzyme A synthetase (EC 6.2.1.1)" in subsystem Ketoisovalerate oxidoreductase or Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 6.2.1.1)

MetaCyc Pathways

• superpathway of coenzyme A biosynthesis II (plants) (9/10 steps found)
• 2-methylcitrate cycle I (5/5 steps found)
• ethanol degradation II (3/3 steps found)
• ethanol degradation IV (3/3 steps found)
• superpathway of acetate utilization and formation (3/3 steps found)
• β-alanine biosynthesis II (5/6 steps found)
• 2-methylcitrate cycle II (5/6 steps found)
• acetate and ATP formation from acetyl-CoA III (1/1 steps found)
• acetate conversion to acetyl-CoA (1/1 steps found)
• L-isoleucine biosynthesis V (2/3 steps found)
• acrylate degradation II (2/3 steps found)
• ethanol degradation III (2/3 steps found)
• L-isoleucine biosynthesis IV (4/6 steps found)
• chitin deacetylation (2/4 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (5/9 steps found)
• adlupulone and adhumulone biosynthesis (1/6 steps found)
• colupulone and cohumulone biosynthesis (1/6 steps found)
• lupulone and humulone biosynthesis (1/6 steps found)
• cis-geranyl-CoA degradation (3/9 steps found)
• superpathway of bitter acids biosynthesis (3/18 steps found)

KEGG Metabolic Maps

• Glycolysis / Gluconeogenesis
• Propanoate metabolism
• Pyruvate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.1, 6.2.1.17

Use Curated BLAST to search for 6.2.1.1 or 6.2.1.17

Protein Sequence (658 amino acids)

>GFF6925 Acetyl-CoA synthetase (EC 6.2.1.1) (Variovorax sp. SCN45)
MNAPDLQPRLPTYAPPQALARTAHVAGRAAYDALVAEAEADHEGYWARLAREFVGWKTPF
IRTLDSSDAPHYRWFEDGTLNVSWNCLDRQVAAGRGDKVAIVFESDDGQVTRTTYAQLLA
QTCRMANALKSLGVRKGDRVVIYMPMSVEGVVAMQACARLGAVHSVVFGGFSAHALRDRV
ADTGATVIVTAAQQVRGGKRLPLKALVDEALALGGCEAVRSVVVHRRTGEPIEWTARDVW
MHEITQAQGDSCEPEWVGAEHPLFLLYTSGSTGKPKGVQHCSGGYLLHAALTTKWTFDMH
DDDVFWCTADIGWVTGHTYIAYGPLAIGATQVVFEGVPTYPDAGRFWQMIERHRVTVFYT
APTAIRSLIKAAEGNPAVHPDRHDLGSLRVLGSVGEPINPAAWEWYRQHVGGGRCPVLDT
WWQTETGGHMITPLPGATDLVPGSCTLPFPGIAAAIVDETGNDVPDGESGLLVIKKPWPS
MIRAVWGDDARYRSSYFPPELRGCYLAGDGAARDRLTRYFTIGGRIDDVLNVSGHRMGTM
EIESALVACAALVAEAAVVGRPDETTGEAISAFVVLKQPRPTGKEGDRLAAELRAWIGKE
IGPIAKPREIRFADNLPKTRSGKIMRRLLRSVARGEAISQDVSTLENPAILDQLTETH