Protein Info for GFF6917 in Variovorax sp. SCN45

Annotation: Uncharacterized MFS-type transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 446 transmembrane" amino acids 39 to 57 (19 residues), see Phobius details amino acids 64 to 89 (26 residues), see Phobius details amino acids 100 to 123 (24 residues), see Phobius details amino acids 129 to 153 (25 residues), see Phobius details amino acids 165 to 187 (23 residues), see Phobius details amino acids 199 to 219 (21 residues), see Phobius details amino acids 243 to 268 (26 residues), see Phobius details amino acids 287 to 305 (19 residues), see Phobius details amino acids 317 to 337 (21 residues), see Phobius details amino acids 350 to 370 (21 residues), see Phobius details amino acids 381 to 402 (22 residues), see Phobius details amino acids 413 to 431 (19 residues), see Phobius details PF00083: Sugar_tr" amino acids 29 to 241 (213 residues), 93.1 bits, see alignment E=1.9e-30 amino acids 246 to 399 (154 residues), 22.3 bits, see alignment E=5.6e-09 PF07690: MFS_1" amino acids 34 to 382 (349 residues), 100.4 bits, see alignment E=1e-32

Best Hits

Swiss-Prot: 67% identical to CITA_ECOLX: Citrate-proton symporter (citA) from Escherichia coli

KEGG orthology group: K03288, MFS transporter, MHS family, citrate/tricarballylate:H+ symporter (inferred from 94% identity to vpe:Varpa_0747)

MetaCyc: 66% identical to propane-1,2,3-tricarboxylate-proton symporter (Salmonella enterica enterica serovar Typhimurium str. LT2)
RXN1R65-49

Predicted SEED Role

"General substrate transporter"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (446 amino acids)

>GFF6917 Uncharacterized MFS-type transporter (Variovorax sp. SCN45)
MNPAPSTAQKGTAAAHASPAPSSKFATVLRVTGGNFMEMFDFFLFGFYATQISKAFFPAG
DEFASLMLTFMTFGAGFLMRPLGAIFLGAYVDRVGRRKGLIVTLALMATGTLLIACVPSY
ATIGLVAPLLVLIGRLLQGFSAGVELGGVSVYLSEMATPGHKGFYVSWQSASQQVAIVVA
AALGYWLNVTFTSQEIGDFYWRVPFFVGCLIVPVLFIIRRSLQETEEFMARKHRPDAREI
FRSMVTNWGLVVAGMMLVSMTTVSFYLITVYTPTFGKAVLHLSTTDALVVTLCVAISNFI
WLPIMGALSDRVGRKPLLILFTVLTILTAYPSLKWLVGAPSFTRMLEVELWLSFLYASYN
GAMVVALTEVMPVNVRTAGFSLAYSLATAVFGGFTPAIATGLIEMTGDKGAPGLWMTAAA
ICGLIATLVLYRRNVNPADARRVPAV