Protein Info for GFF6899 in Variovorax sp. SCN45

Annotation: Biofilm PGA synthesis deacetylase PgaB (EC 3.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 689 signal peptide" amino acids 1 to 34 (34 residues), see Phobius details TIGR03938: poly-beta-1,6-N-acetyl-D-glucosamine N-deacetylase PgaB" amino acids 47 to 665 (619 residues), 868 bits, see alignment E=1.6e-265 PF01522: Polysacc_deac_1" amino acids 106 to 272 (167 residues), 75.2 bits, see alignment E=4.6e-25 PF14883: GHL13" amino acids 325 to 645 (321 residues), 470.9 bits, see alignment E=2.1e-145

Best Hits

KEGG orthology group: K11931, biofilm PGA synthesis lipoprotein PgaB [EC: 3.-.-.-] (inferred from 54% identity to mms:mma_2648)

Predicted SEED Role

"Biofilm PGA synthesis deacetylase PgaB (EC 3.-)"

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.-.-.-

Use Curated BLAST to search for 3.-.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (689 amino acids)

>GFF6899 Biofilm PGA synthesis deacetylase PgaB (EC 3.-) (Variovorax sp. SCN45)
MRTTDDNPMTFLRAAMLWLVLAAASALALPAFAQPLPPADPDDGLSFRVLSFHDVRTGVR
ASFEQSPDETAIDDSTLAEVFAWLQFSGYHPVSLQQIVDARAGGKPLPARPVLLTFDDGY
RSAYTKVFPLLKRYNYPALFALVTSWLDVPEGQLVHWGDKPAPRENFLLWPEVAEMARSG
LAEFASHTDAMHTGILANPQGNMLPAAATHRYDPKTGRYEDDDAYVRRVEADLRRSREII
EARTGARVRATVWPYGAYNTAALEASARAGMPITFTLDDGANSPDVPLTRIRRALAAYDN
KAPEYAQLLRNPVGGEVRQVNRVMHVDLDYVYDPDPAQQERNLSALIDRVAAVRPRSVFL
QAYADPDGDGVANALYFPNRHLPMRADLFGRAAWQLRTRTGVKVYAWMPVTAFRLPASNP
LATHTVMAQGGKMPADRYHRLTPFDPAVRTLVGDIYEDLGRYTFFEGVLFHDDATLSDDE
DASPSALATYAKWGLPPDVAAIRADPALMARWTAAKTRYLIDFTHELAARLGAWRPALET
ARNLYARPVLEPQAEQWFAQNYEASLAAYDYVALMAMPRMEQEADADAWLGRLARRAADT
PRGLDGTVFELQARDWRTGKPVADAELARQWTLLQRAGVRHLGYYPDDFLNNQPSLDVVR
RAISVRTMLPRALGASAAETTPPQGERAP