Protein Info for GFF687 in Methylophilus sp. DMC18

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 340 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details PF02563: Poly_export" amino acids 21 to 94 (74 residues), 69.6 bits, see alignment E=3.1e-23 PF22461: SLBB_2" amino acids 100 to 176 (77 residues), 33.8 bits, see alignment E=4.6e-12 amino acids 265 to 334 (70 residues), 36.6 bits, see alignment E=6.1e-13 PF10531: SLBB" amino acids 102 to 144 (43 residues), 28.5 bits, see alignment 1.7e-10 amino acids 266 to 313 (48 residues), 58 bits, see alignment 1e-19

Best Hits

KEGG orthology group: K01991, polysaccharide export outer membrane protein (inferred from 44% identity to meh:M301_0751)

Predicted SEED Role

"Capsular polysaccharide biosynthesis/export periplasmic protein WcbA; Capsular polysaccharide export system protein KpsC"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (340 amino acids)

>GFF687 hypothetical protein (Methylophilus sp. DMC18)
MKLLTRCLFLFTMLFPIWAFADYTLGTGDFVKVTVYGDAELTREIRIAENGILTFPLIGE
VKVGGLSTVEAEKKIAEQLQRGGFIASPQVSVVVIQFLSKTVSVLGGVLKPGRYPVTRPS
DVKDMIAEAGGITPDGSEIVTVVRGDKRTELDLHDVINQHASKEEITVAGGETIYVGSRD
VAVVGQLLRPAKYGIQGGMRKISDFISVAGGPTEFAGETIFYTTSISGTPVTEQINIDEL
FKAPNSAHNKQVYPGDVLYVPKAPQVYVYGEVQRPGMYKVDKNMTVMQAIAKAGGLTMRG
TQRSVKLHRKDEHNEVVKQTPSLTSVLQDEDVLYIEESLL