Protein Info for GFF683 in Methylophilus sp. DMC18

Annotation: tRNA dimethylallyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 317 TIGR00174: tRNA dimethylallyltransferase" amino acids 12 to 299 (288 residues), 320.7 bits, see alignment E=4.1e-100 PF01745: IPT" amino acids 15 to 67 (53 residues), 22.1 bits, see alignment 9.2e-09 PF01715: IPPT" amino acids 46 to 290 (245 residues), 285.9 bits, see alignment E=3.1e-89

Best Hits

Swiss-Prot: 62% identical to MIAA_METFK: tRNA dimethylallyltransferase (miaA) from Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875)

KEGG orthology group: K00791, tRNA dimethylallyltransferase [EC: 2.5.1.75] (inferred from 61% identity to mmb:Mmol_0844)

MetaCyc: 56% identical to tRNA dimethylallyltransferase (Escherichia coli K-12 substr. MG1655)
RXN0-6274 [EC: 2.5.1.75]

Predicted SEED Role

"tRNA dimethylallyltransferase (EC 2.5.1.75)" (EC 2.5.1.75)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.5.1.75

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (317 amino acids)

>GFF683 tRNA dimethylallyltransferase (Methylophilus sp. DMC18)
MPAPDKSHLPPAIFFMGPTASGKTAATIALAQRFPVEIISVDSALVYRGMDIGTAKPDAE
TLRQAPHHLIDLITPLEQYSAAQFAKDALALMHAATANGRIPVLVGGTMLYFNALQHGLS
DLPPADPVIRAQIEAEASAVGWPVMHAQLASIDPSVAAKIHTTDSQRIERALEVYRISGQ
PMSVLRAQSEKVTLPFDILKIALMPSDRSVLHQRIADRFQAMLKQGFIEEVQGLLSAYPQ
LTTETPSMRCVGYRQVLQHLNGEIDLPTLADHGVYATRQLAKRQLTWLRGMDDVEVLDCL
SPSWIGQMLTRMTAHSD