Protein Info for GFF680 in Xanthobacter sp. DMC5

Annotation: Putative L-lactate dehydrogenase operon regulatory protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 260 PF00392: GntR" amino acids 14 to 72 (59 residues), 52.2 bits, see alignment E=3.9e-18 PF07729: FCD" amino acids 105 to 232 (128 residues), 96.3 bits, see alignment E=1.9e-31

Best Hits

Swiss-Prot: 34% identical to GLCC_ECOL6: Glc operon transcriptional activator (glcC) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K14348, GntR family transcriptional regulator, L-lactate dehydrogenase operon regulator (inferred from 55% identity to rva:Rvan_0889)

Predicted SEED Role

"Lactate-responsive regulator LldR in Enterobacteria, GntR family" in subsystem Lactate utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (260 amino acids)

>GFF680 Putative L-lactate dehydrogenase operon regulatory protein (Xanthobacter sp. DMC5)
MAPSPSPSSAQRVADRVELAIEALIEARGLAVGDRLPSERALSEMLGASRNTLREALMRL
GARGRITIRKGGNIIAEPAEARWTAATIADPLASVVAGNPGFGHDVLEVRRGLEGQAAYH
AALRATPDDLDAIRRCIDQLSAYHEKGDAVAEARADAAFHLAIADASHNAVLRSVMSSMF
GLLQSSISESLDKLYIVPRTYERLSAQHETLMDTIAAGDADAARRASDDHLAFVAQTIRT
IDEERARRERADSLRRTARI