Protein Info for GFF678 in Variovorax sp. SCN45

Annotation: Error-prone repair homolog of DNA polymerase III alpha subunit (EC 2.7.7.7)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1117 TIGR00594: DNA polymerase III, alpha subunit" amino acids 36 to 933 (898 residues), 508.4 bits, see alignment E=2.3e-156 PF02811: PHP" amino acids 37 to 159 (123 residues), 47.1 bits, see alignment E=9e-16 PF07733: DNA_pol3_alpha" amino acids 332 to 589 (258 residues), 293.5 bits, see alignment E=4.2e-91 PF17657: DNA_pol3_finger" amino acids 592 to 760 (169 residues), 184.1 bits, see alignment E=3.4e-58 PF14579: HHH_6" amino acids 834 to 942 (109 residues), 64.3 bits, see alignment E=2.5e-21 PF01336: tRNA_anti-codon" amino acids 1024 to 1085 (62 residues), 30.8 bits, see alignment 5.8e-11

Best Hits

Predicted SEED Role

"DNA polymerase III alpha subunit (EC 2.7.7.7)" in subsystem DNA-replication (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1117 amino acids)

>GFF678 Error-prone repair homolog of DNA polymerase III alpha subunit (EC 2.7.7.7) (Variovorax sp. SCN45)
MPELSGKQESRRQPAKVHALPVPLRRPPALTLPDYAELHCLTNFSFQRGASTPEEMVERA
YQLGYKALAITDECSVAGIVRAHVCLRGMEHKLDEYEREHPDEPKIPRNPTFRLLFGSEF
QFERFRLVVIANDTEGWGNLCEFITAARNTELPKGEYRVGWEESDVASLQHCQILFVPNR
NPGGAMDKATLHEDLLAARALYGENLWLAVELFNELDDDLWLVTLMEVGEQAGVPLVAAG
DVHMHARSCKPLHDVLTAVREGKTVAECGFALQSNAQRHLRPRMRLAEIHLRRMLENTLA
VAGRCNFDPEVIRENYKYPLETLGSDETPAQTLVRKTWEGARGRYPEGIPDKVRAQVQKE
LDIIIDLKYEMFFLTVENIVSFARSQKILCQGRGSSANSAVCFCLGITAIDPTKGHLLFE
RFLSRERHEPPDIDVDFEHQRREEVIQYIYAKYGRHRAAIAAVVICYRSRSALRDVGKAI
GIDERLIDEFAKDHYWFDDTVLGEQLRQAQARVGVVEDELKLVHWIEMTQKLKGFPRHLS
QHVGGFVLTHTRLTRLVPVEKASMKDRSVIQWEKDDLEAMGMLKVDVLALGMLSAIRRGL
EHMNRWRGSTIEMHQIPNDDQKVFDMICDADTIGVFQIESRAQMSMLPRLKPRTYEDLVI
EVAIVRPGPIQGGMVHPYLKQRERVRKGLPIHYEKEELREALERTLGIPIFQEQVMQIAM
IAAKFTADEADQLRRAMAAWKRKGGLGKFHDKLVNGMTLNGYKASFAEAIFKQVMGFGDY
GFPESHAASFALLVTVSSWLKNYEPACFLAALLDSQPMGFYSPSQLVQDARRHGVEVRPV
DVTRSDFDTTLEAREPDAPRPSGIDERYADRLGNENQPAVRLGLNRIAGFSAGGVERLLK
ARAAAPFTSTEDLALRAELEGKDMAALAAADALMSLSGHRRQQVWDATAQRRAPALLRGV
PINEQALLLPAASEGEEIVGDYASLRLTLRRHPLALLRPRLARMKLMSAAELRSVPNGQT
ARACGIVKGRQRPQTANGTIFVTLEDETGNVNVIVWSHVIEAWREPLLKSHLLAVQGTWQ
RDDETGGKVQHLVATGFKDLTPLMGRLAQSNTSRDFH