Protein Info for GFF6779 in Variovorax sp. SCN45

Annotation: ATP-dependent RNA helicase RhlE (EC 3.6.4.13)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 601 PF00270: DEAD" amino acids 25 to 198 (174 residues), 163.9 bits, see alignment E=4.5e-52 PF04851: ResIII" amino acids 26 to 193 (168 residues), 28.6 bits, see alignment E=1.9e-10 PF00271: Helicase_C" amino acids 234 to 342 (109 residues), 100.3 bits, see alignment E=1.1e-32

Best Hits

KEGG orthology group: K11927, ATP-dependent RNA helicase RhlE [EC: 3.6.4.13] (inferred from 93% identity to vap:Vapar_3682)

Predicted SEED Role

"ATP-dependent RNA helicase RhlE" in subsystem ATP-dependent RNA helicases, bacterial

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.13

Use Curated BLAST to search for 3.6.4.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (601 amino acids)

>GFF6779 ATP-dependent RNA helicase RhlE (EC 3.6.4.13) (Variovorax sp. SCN45)
MNFDELKLAPAILKAVHEHGYDTPTPIQAQAIPAVLDGHDLLGGAQTGTGKTAAFTLPML
HKLSMSRSATNKFGGTGIRALVLTPTRELAAQVEESVRTYGKYLQLDSTVIFGGVGMNPQ
ISKLKKGVDILVATPGRLLDLQQQGMLDLSQVQMLILDEADRMLDMGFIHDVKKILALVP
KEKQSLLFSATFSDDIRDLASTLLKNPQSIQVTPRNTTVQRITQVIHPVGRGKKKALLAH
IINENKWSQVLVFTRTKFGANSVAEFLTKNGIEAMALHGNKSQSARTQALAGFKSGDIRA
LVATDIAARGIDIDELPHVVNYEIPNVSEDYVHRIGRTGRAGSSGEAVSFVCLDEEGFMQ
EIERFTKQTIPVQIVEGFGPEEGERAEPIAMGRQTIWGGAGRPPSRDVMQAAAKAARTEM
LQRIRENKAGQGGGNGGGGGNGGGQRRGGGQGGGQGRGANGGGQGQGQGPRGPGGGGARA
PQGRGPQGPARAPHHAPHQQAHQPHDERQPRHHGNSHSPTQANQVAHLRAEAVGGGAGQP
DPLRTSVDHMGGGRGRGRPGGGGGGYGGGNRSGGGGRSGGGGGYGGGGGRSGGGGGRSFG
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