Protein Info for GFF672 in Methylophilus sp. DMC18

Annotation: Bicyclomycin resistance protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 385 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details transmembrane" amino acids 31 to 52 (22 residues), see Phobius details amino acids 64 to 80 (17 residues), see Phobius details amino acids 86 to 107 (22 residues), see Phobius details amino acids 119 to 139 (21 residues), see Phobius details amino acids 144 to 144 (1 residues), see Phobius details amino acids 146 to 165 (20 residues), see Phobius details amino acids 197 to 221 (25 residues), see Phobius details amino acids 234 to 252 (19 residues), see Phobius details amino acids 264 to 286 (23 residues), see Phobius details amino acids 293 to 320 (28 residues), see Phobius details amino acids 335 to 354 (20 residues), see Phobius details amino acids 360 to 378 (19 residues), see Phobius details TIGR00710: drug resistance transporter, Bcr/CflA subfamily" amino acids 1 to 378 (378 residues), 228.8 bits, see alignment E=7.3e-72 PF07690: MFS_1" amino acids 3 to 344 (342 residues), 148 bits, see alignment E=1.8e-47

Best Hits

KEGG orthology group: K07552, MFS transporter, DHA1 family, bicyclomycin/chloramphenicol resistance protein (inferred from 45% identity to azo:azo3735)

Predicted SEED Role

"MFS transporter"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (385 amino acids)

>GFF672 Bicyclomycin resistance protein (Methylophilus sp. DMC18)
MLAALAPFAIDTYLPAFHVMAAEFGTDELAIQQSLTLYLLPYAVMTLCHGAISDAIGRIT
TIKWGLAIFMLASLGCAFAPNVQTLWLFRALQGVSGGAGNTVARAMVRDLFDGPQAQRVM
ATVQLLFGIAPAVAPILGGVLLGIHWQAIFLFLALYAAVALWMAVRHLPETVSPEKRIAF
SVAGIAHTYGQMLKNRLFLCLITCLGLNFSAFFIYVLASPVFLVKHLHLNSQQFGYLFIP
TVTGMMLGSWISRYTAGKVAAARLLRWAFAWMVAIAILNVCVQVAASDQLHEAWWLAILP
IALFNIGMATAMPVLSIAALDCYPKLRGTAASAQAFMQMLCSTLSSALVVPLVWHSPLTL
ASAMLAMTGVSALLLFFVRRLKVSA