Protein Info for GFF6716 in Variovorax sp. SCN45

Annotation: N-acyl-D-amino-acid deacylase (EC 3.5.1.81)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 493 PF07969: Amidohydro_3" amino acids 60 to 229 (170 residues), 47.7 bits, see alignment E=1.8e-16 amino acids 360 to 472 (113 residues), 49.5 bits, see alignment E=4.9e-17

Best Hits

Swiss-Prot: 50% identical to NDAD_ALCXX: D-aminoacylase (dan) from Alcaligenes xylosoxydans xylosoxydans

KEGG orthology group: K06015, N-acyl-D-amino-acid deacylase [EC: 3.5.1.81] (inferred from 95% identity to vpe:Varpa_1303)

Predicted SEED Role

"D-aminoacylase (EC 3.5.1.81)" (EC 3.5.1.81)

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.81

Use Curated BLAST to search for 3.5.1.81

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (493 amino acids)

>GFF6716 N-acyl-D-amino-acid deacylase (EC 3.5.1.81) (Variovorax sp. SCN45)
MADNNTSSKAVLLEGGLVVDGSGGPSWPGDVLLQGDRIAALGEGLRERLPQGLALADIDI
VDCRAKVIAPGFIDAHTHDDAIVLRDPLCLPKVSQGITTVVTGNCGISLAPYRTPQSLPP
LTLLGADSFKHATMAEYRAAVDATQPALNVAALVGHTTLRFAAMEALDRPASGDELARME
ALLDSCMADGAHGMSSGLFYEEAFAAPAEEVTALARVVARHGGVYATHLRSEMQQIIEAL
HEAGDSAFAAGVPLVISHHKCAGPANWGRTRETLPLIESLAQRQKIAMDVYPYVAGSTVL
REDLVDGVIDVLLTWSDPHPEMTGRLISDIAREWGTTEQEACLRLKPGGACYFQMQEEDV
ERVIAHPLTMIGSDGLPHDRHPHPRLWGAFPRVFARYWRQRRLFTLEQAVHKMTGMTARN
LRIADRGLLRAGNMADVVVFDPETIADTATYDQPHGVSLGVERVFVNGVLAYRGGEPEAR
VLARAGRMLTRGA