Protein Info for GFF6700 in Variovorax sp. SCN45

Annotation: Single-stranded-DNA-specific exonuclease RecJ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 575 TIGR00644: single-stranded-DNA-specific exonuclease RecJ" amino acids 22 to 570 (549 residues), 429.1 bits, see alignment E=1e-132 PF01368: DHH" amino acids 73 to 206 (134 residues), 76.3 bits, see alignment E=3.9e-25 PF02272: DHHA1" amino acids 320 to 456 (137 residues), 57.2 bits, see alignment E=3.9e-19 PF17768: RecJ_OB" amino acids 475 to 571 (97 residues), 62.3 bits, see alignment E=6.3e-21

Best Hits

KEGG orthology group: K07462, single-stranded-DNA-specific exonuclease [EC: 3.1.-.-] (inferred from 94% identity to vpe:Varpa_1288)

Predicted SEED Role

"Single-stranded-DNA-specific exonuclease RecJ (EC 3.1.-.-)" in subsystem DNA-replication or DNA Repair Base Excision or DNA repair, bacterial RecFOR pathway (EC 3.1.-.-)

Isozymes

Compare fitness of predicted isozymes for: 3.1.-.-

Use Curated BLAST to search for 3.1.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (575 amino acids)

>GFF6700 Single-stranded-DNA-specific exonuclease RecJ (Variovorax sp. SCN45)
MKIIDREIPPRTVWALEQAGVHPLLARLFAARGVRAKDELDDGLARLLSPDTLRGTHDAA
VLLADAIAQDKRLCIVADYDCDGATACAVGVRGLRLLGAKNVSYLVPDRVVDGYGLTPPI
AERVAASGADMLITVDNGIASVEGVAAAKARGLQVLVTDHHLPGPELPLADVLVNPNQPG
CEFESKSIAGVGVMFYVLLALRSELRKRGVFDTATQPKLDALLPLVALGTVADVVKLDAN
NRRLVAQGLRRIRAGAMPAGVAALFKAASRHASAATTFDFGFALGPRINAAGRLADMTLG
IECLLTDDAGRAEELARMLDGINRERRDIEGGMRDQALLLAESLFSNGTDGEGIAAPPPA
ISVFDAGFHEGVVGIVASRIKDRFHRPTFVFAASGAPGKEQELKGSGRSIPGFHLRDALD
LVAKRHPGVLLRFGGHAMAAGCTVAREHFETFEKGFAQVARELLADAALTRQIETDGPIA
REYMRIDLVDTLHREVWGQGFAPPTFSEEVEVVSQRLVGEKHLSLKLRHQGQPIDAMWFS
HTEPLPPRVKLAFRLDADEWQGVRRLRFLVEGAEF