Protein Info for PS417_03345 in Pseudomonas simiae WCS417

Annotation: type I secretion protein TolC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 406 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF02321: OEP" amino acids 28 to 202 (175 residues), 80.8 bits, see alignment E=5.7e-27 amino acids 231 to 400 (170 residues), 98 bits, see alignment E=3.2e-32

Best Hits

KEGG orthology group: None (inferred from 88% identity to pfs:PFLU0697)

Predicted SEED Role

"Heavy metal RND efflux outer membrane protein, CzcC family" in subsystem Cobalt-zinc-cadmium resistance

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7U1Q8 at UniProt or InterPro

Protein Sequence (406 amino acids)

>PS417_03345 type I secretion protein TolC (Pseudomonas simiae WCS417)
MAISVKKLLGAGVLWVVASAAQAQTLTLDSALQTAFANNPDLAAAQWEIDIAQGGRQQAG
LIPNPVASWDVEDTRRDSRTTSIKISQALELGGKRGARIDVASRVQDAAALTLEQRRNSL
RAEVIDNYYGALRAQERLDLAQRSMTLAERGLVVANGRVTAGKASPVEATRAQVQLSEIR
LERDRAQMGLTDAYRRLAASTGSAAPNFQAVATRNPSTLAVPSATQLLARLEQTAELRLA
ELSILQYEASIGLEKAQRIPDLDVSIGSQYDASVRERVSLVGVSMPIPLFNRNQGNVLAA
TRRADQARDLRNATELRLRTETRQAVDLWQSANTEVRVFNQQILPAAQSAVDSATRGFEM
GKFSFLDVLDAQRTLIAARTQYLTATAQATDAWVRIERIYGDLARF