Protein Info for GFF658 in Pseudomonas sp. DMC3

Annotation: Gluconate 2-dehydrogenase flavoprotein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 594 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF00732: GMC_oxred_N" amino acids 234 to 348 (115 residues), 29.1 bits, see alignment E=8.8e-11 PF05199: GMC_oxred_C" amino acids 451 to 572 (122 residues), 71.1 bits, see alignment E=1.5e-23

Best Hits

Swiss-Prot: 77% identical to GADH1_PANCY: Gluconate 2-dehydrogenase flavoprotein from Pantoea cypripedii

KEGG orthology group: K06151, gluconate 2-dehydrogenase alpha chain [EC: 1.1.99.3] (inferred from 98% identity to pfo:Pfl01_0085)

MetaCyc: 95% identical to D-gluconate dehydrogenase dehydrogenase subunit (Pseudomonas fluorescens)
Gluconate 2-dehydrogenase (acceptor). [EC: 1.1.99.3]

Predicted SEED Role

"Gluconate 2-dehydrogenase (EC 1.1.99.3), membrane-bound, flavoprotein" in subsystem D-gluconate and ketogluconates metabolism (EC 1.1.99.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.99.3

Use Curated BLAST to search for 1.1.99.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (594 amino acids)

>GFF658 Gluconate 2-dehydrogenase flavoprotein (Pseudomonas sp. DMC3)
MATVMKKVDAVIVGFGWTGAIMAKELTEAGLNVLALERGPMQDTYPDGNYPQVIDELTYS
VRKKLFQDISKETVTIRHSVNDIALPNRQLGAFLPGNGVGGAGLHWSGVHFRVDPIELRM
RSHYEERYGKSFIPKDMTIQDFGVSYEELEPFFDFAEKVFGTSGQAWTVKGQLVGQGKGG
NPYAADRSNPFPLEAQKNTVSAQLFGKAATEVGYKPYNLPSANTSGPYTNPYGAQMGPCN
FCGFCSGYVCYMYSKASPNVNILPALKPLPNFELRPNSHVLRVNLDSSKSKATGVTYIDG
QGREIEQPADLVILGAFQLHNVRLMLLSGIGKPYDPISGEGVVGRNFAYQNMATIKAFFD
KDTHTNNFIGAGGNGVAVDDFNADNFDHGPHGFVGGSPMWVNQAGSRPIAGTSNPPGTPA
WGSAWKRATADYYTHQVSMDAHGAHQSYRGNYLDLDPVYRDAYGLPLLRMTFDWQENDIK
MNRFMVEKMGKIAQAMGPKAIAVIGKQVGDHFNTASYQTTHLNGGAIMGTDPKTSALNRY
LQSWDVHNVFVPGASAFPQGLGYNPTGLVAALTYWSAKAIREQYLKNPGPLVQA