Protein Info for GFF656 in Sphingobium sp. HT1-2

Annotation: Lysyl-tRNA synthetase (class I) (EC 6.1.1.6)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 526 PF01921: tRNA-synt_1f" amino acids 15 to 367 (353 residues), 464.1 bits, see alignment E=1.4e-143 TIGR00467: lysine--tRNA ligase" amino acids 41 to 522 (482 residues), 176.6 bits, see alignment E=3.7e-56

Best Hits

Swiss-Prot: 58% identical to SYK_CAUSK: Lysine--tRNA ligase (lysS) from Caulobacter sp. (strain K31)

KEGG orthology group: K04566, lysyl-tRNA synthetase, class I [EC: 6.1.1.6] (inferred from 90% identity to sch:Sphch_0021)

Predicted SEED Role

"Lysyl-tRNA synthetase (class I) (EC 6.1.1.6)" (EC 6.1.1.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (526 amino acids)

>GFF656 Lysyl-tRNA synthetase (class I) (EC 6.1.1.6) (Sphingobium sp. HT1-2)
MTISDILRTAANASKAWPYEEARKLLKRYPNGAPEKGHILFETGYGPSGLPHIGTFNEVL
RTTMVRNAYHALSDIPTRLVAFSDDLDGFRKVPDNVPNQAMLAEYLGKPLTQVPDPFEKY
ESFAHHNNAMLREFLDSFGFDYEFVSATEQYRAGAFDEALRQVLRRYQAIMDIMLPTLRK
ERQATYSPVLPISPKSGIVLQVPVEVVDAEAGIVRFVDEGETIEQSILGGGAKLQWKVDW
AMRWYALGVDYEMAGKDLIDSVTQSSKICRALGGRPPEGFNYEMFLDEKGEKISKSKGNG
LSLEQWLTYGPQESLAFYAYREPKKAKQLHMGVIPRAVDEYWQFRGNYPKQAIEQQLGNP
VHHIHDGKLPQGELPVTFGLLLNLVGVMGEASREQVWSYLQNYVPGASAETYPELDALIG
HALAYHRDFVAPTLQRRAPHANEAAALAKLDEELAALPADASAEDIQNIVYAIGKEEAFG
FAELRDWFKALYQTLLGADQGPRMGSFIALYGIDNSRKLIAQALAA