Protein Info for GFF656 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Quino(hemo)protein alcohol dehydrogenase, PQQ-dependent (EC 1.1.99.8)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 712 signal peptide" amino acids 1 to 30 (30 residues), see Phobius details TIGR03075: PQQ-dependent dehydrogenase, methanol/ethanol family" amino acids 49 to 578 (530 residues), 677 bits, see alignment E=9.2e-208 PF13360: PQQ_2" amino acids 89 to 139 (51 residues), 22.7 bits, see alignment 1.5e-08 amino acids 122 to 248 (127 residues), 60.1 bits, see alignment E=5.2e-20 amino acids 490 to 571 (82 residues), 33.9 bits, see alignment E=5.4e-12 PF13570: PQQ_3" amino acids 92 to 131 (40 residues), 20.7 bits, see alignment 8.9e-08 amino acids 132 to 182 (51 residues), 23.2 bits, see alignment 1.5e-08 amino acids 505 to 542 (38 residues), 20.9 bits, see alignment 8.1e-08 PF01011: PQQ" amino acids 113 to 141 (29 residues), 23.4 bits, see alignment (E = 7.4e-09) amino acids 164 to 191 (28 residues), 28 bits, see alignment (E = 2.7e-10) amino acids 524 to 559 (36 residues), 31.1 bits, see alignment (E = 2.7e-11) PF13442: Cytochrome_CBB3" amino acids 629 to 701 (73 residues), 40.3 bits, see alignment E=6.7e-14

Best Hits

Swiss-Prot: 74% identical to QHED_COMTE: Quinohemoprotein alcohol dehydrogenase (qheDH) from Comamonas testosteroni

KEGG orthology group: K00114, alcohol dehydrogenase (cytochrome c) [EC: 1.1.2.8] (inferred from 78% identity to pol:Bpro_5302)

MetaCyc: 74% identical to alcohol dehydrogenase (azurin) (Comamonas testosteroni)
RXN-11335 [EC: 1.1.9.1]

Predicted SEED Role

"Quino(hemo)protein alcohol dehydrogenase, PQQ-dependent (EC 1.1.99.8)" (EC 1.1.99.8)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.2.8, 1.1.99.8

Use Curated BLAST to search for 1.1.2.8 or 1.1.9.1 or 1.1.99.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (712 amino acids)

>GFF656 Quino(hemo)protein alcohol dehydrogenase, PQQ-dependent (EC 1.1.99.8) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MKPTPLVSVRCRAAACLLAALTLGGAAFAQLPAPIKGSQEHIRAAVGKVNATFMKANAGK
TPDWPSYGLDYAETRHSKLNQISTANVKDLGLVWSYNLESSRGVESTPLVVDGIMYVTAS
WSVVHAVDVRTGKRLWTFDPKVSREAGYKGCCDVVNRGVALHEGKVFVAAYDGRLIAIDA
ATGEQVWSKDTIIDRKFSYTITGAPRVFKGKVIIGNGGAEYGVRGYITAYDAKTGDQKWR
WFTVPGDPSKPFEDESMAKAAKTWDPAGKWWEAGGGGTAWDTMTFDPELNLMYIGTGNGS
PWARSKRSPAGGDNLYLASIVALNPDTGKYVWHYQETPGDNWDFTSTQPMILADIKLDNK
PRKVILHAPKNGFFFVIDRTNGKFISAKNFVDVNWATGYDKNGRPIETPAARTADRPREI
IPSAFGARNWHSMSYNPATGLVYMPVQSVPLTLMDNKDWKFNADRPGEPHANMGWNLATF
ANVEPPTSKPFGRLVAWDPVQQKARWTQEQISPWNGGTLTTAGNLVFQGTADGRFIAYNA
TSGEKLWETPTGTGVIAAPSTYMVDGKQYVSIAVGWGGVYGLAQRATDRQGPGTVYTFAV
GGKAQPPAFVNYQLGKLVAGVKYDPAHVPAGTALYVSNCVFCHGVPGVDRGGNIPNLGYM
DSAFIENLDKFVFAGPAMARGMPDFTGKLSQDDVQKIKAFIQGTADAIRPKN