Protein Info for GFF6544 in Variovorax sp. SCN45

Annotation: D-Lactate dehydrogenase, cytochrome c-dependent (EC 1.1.2.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 468 PF01565: FAD_binding_4" amino acids 51 to 187 (137 residues), 126.6 bits, see alignment E=5.7e-41 PF02913: FAD-oxidase_C" amino acids 225 to 465 (241 residues), 231.6 bits, see alignment E=1.2e-72

Best Hits

Swiss-Prot: 50% identical to LDHD_MOUSE: Probable D-lactate dehydrogenase, mitochondrial (Ldhd) from Mus musculus

KEGG orthology group: None (inferred from 86% identity to vpe:Varpa_4310)

Predicted SEED Role

"D-Lactate dehydrogenase, cytochrome c-dependent (EC 1.1.2.4)" in subsystem Glycolate, glyoxylate interconversions or Photorespiration (oxidative C2 cycle) (EC 1.1.2.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.2.4

Use Curated BLAST to search for 1.1.2.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (468 amino acids)

>GFF6544 D-Lactate dehydrogenase, cytochrome c-dependent (EC 1.1.2.4) (Variovorax sp. SCN45)
MDTVVSSSTRRSPSPELLALLEREFAERFSISRAVLQQHGTDESTYPPQPPDAVVFVCST
EEAAFVVRACAKERVPVIGFGVGSSVEGHLLAVQGGVCIDFSRMNKVLAIRPGDLTATVE
AGVTRGQLNAALQDTGFFFSVDPGADASIGGMVATAASGTNTVRYGTMRDNLVSLTVITA
DGNVVRTASRARKSSAGYNLTQLYCGSEGTLGLITEATVRLHPHPEAYAAAAVNFPSVRA
AVDCVIESIQMGVPLARAEMLDALTIRAVNAHSHTTLGESPTLFLEFGASRTQIEEQAGI
VREIAAGHGGGEFQWANRQEDRSRLWTPRHHAYFACLQLKAGSRSLTTDACVPLSALAQC
IDETQADIAEHGLLAPVFGHVGDGNFHCLVLVDPNSAEENEAAEAFSHRLVQRALRHGGT
CTGEHGVGMHKMKYLAEEHGEHGVALMRSVKQALDPHDIFNPGKVVGV