Protein Info for GFF6520 in Variovorax sp. SCN45

Annotation: Pyridoxamine 5'-phosphate oxidase (EC 1.4.3.5)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00558: pyridoxamine 5'-phosphate oxidase
  • amino acids 9 to 217 (209 residues), 272.4 bits, E=1.3e-85
• PF01243: PNPOx_N
  • amino acids 39 to 161 (123 residues), 98.2 bits, E=4.3e-32
• PF10590: PNP_phzG_C
  • amino acids 177 to 217 (41 residues), 70.3 bits, 1e-23

Best Hits

Swiss-Prot: 77% identical to PDXH_ACIAC: Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) from Acidovorax citrulli (strain AAC00-1)

KEGG orthology group: K00275, pyridoxamine 5'-phosphate oxidase [EC: 1.4.3.5] (inferred from 97% identity to vpe:Varpa_4289)

MetaCyc: 34% identical to PhzG monomer (Pseudomonas fluorescens)
RXN-12021 [EC: 1.10.3.16]; 1.10.3.16 [EC: 1.10.3.16]; 1.10.3.16 [EC: 1.10.3.16]; 1.10.3.16 [EC: 1.10.3.16]

Predicted SEED Role

"Pyridoxamine 5'-phosphate oxidase (EC 1.4.3.5)" in subsystem Pyridoxin (Vitamin B6) Biosynthesis (EC 1.4.3.5)

MetaCyc Pathways

• pyridoxal 5'-phosphate biosynthesis I (6/7 steps found)
• phenazine-1-carboxylate biosynthesis (12/17 steps found)
• superpathway of pyridoxal 5'-phosphate biosynthesis and salvage (7/12 steps found)
• pyridoxal 5'-phosphate salvage I (2/6 steps found)
• 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis II (2/8 steps found)
• pyridoxal 5'-phosphate salvage II (plants) (2/9 steps found)

KEGG Metabolic Maps

• Vitamin B6 metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.10.3.16 or 1.4.3.5

Protein Sequence (217 amino acids)

>GFF6520 Pyridoxamine 5'-phosphate oxidase (EC 1.4.3.5) (Variovorax sp. SCN45)
MTSPLSNDALAALRKSYERAELGEAHSEGDPLKQFERWLTEAIDARVPEPNAMTLCTVGS
DLRPSSRIVLIKGYDARGIVWYTNYESRKGRELSGNPYASLQFHWVELERVVRIEGRVEK
AGADESDAYFASRPLDSRIGAWASPQSEVISGRDVLVKNAAVFAAKHLLSPPRPPHWGGY
RLVPDRWEFWQGRKSRLHDRLRYRLEGADWVRERLAP