Protein Info for GFF6513 in Variovorax sp. SCN45

Annotation: Phosphogluconate dehydratase (EC 4.2.1.12)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 610 TIGR01196: phosphogluconate dehydratase" amino acids 6 to 608 (603 residues), 891.8 bits, see alignment E=9.5e-273 PF00920: ILVD_EDD" amino acids 70 to 606 (537 residues), 514.1 bits, see alignment E=2.1e-158

Best Hits

Swiss-Prot: 58% identical to EDD_ZYMMO: Phosphogluconate dehydratase (edd) from Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4)

KEGG orthology group: K01690, phosphogluconate dehydratase [EC: 4.2.1.12] (inferred from 95% identity to vpe:Varpa_4283)

MetaCyc: 56% identical to phosphogluconate dehydratase (Escherichia coli K-12 substr. MG1655)
Phosphogluconate dehydratase. [EC: 4.2.1.12]

Predicted SEED Role

"Phosphogluconate dehydratase (EC 4.2.1.12)" in subsystem Entner-Doudoroff Pathway (EC 4.2.1.12)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (610 amino acids)

>GFF6513 Phosphogluconate dehydratase (EC 4.2.1.12) (Variovorax sp. SCN45)
MSSSTHPTVRDVTDRIRERSHGLRSAYLAQVAEIRGRDRGSDRMGCANVAHAVAGIPAND
KFRVVAERAPNIGIVTAYNDMLSAHAPYQGYPDIIKQEARSLGATAQVAGGVPAMCDGVT
QGTPGMELSLFSRDVIAMSTAVALTHDMFDAALMLGVCDKIVPGLLIGALHFGHLPTVFV
AAGPMPSGLSNGAKSKVREQAAQGLVGRQGLLEAEMAAYHTLGTCTFYGTANSNQMLLEA
MGLHVPGTAFIQPGDAMRETLTREAVRTVLGKAGDAAFNCPPIGEMVDERCIVNAMVALL
ATGGSTNHLIHWVAVARAAGIVIDWDDFSKLSDIIPLLTRVYPNGSADVNEFQAAGGPGF
VIGELVDAGLMHGDVLTVRAGGIREFANVPELAGEQRLRWTPAAPSKNDAVARPVSSPFS
ATGGLKLLSGNLGRSVIKVSSVPDDRHVVEAPARVFDSQAALHKAFTAGELERDVVCVVR
WQGPQANGMPELHKLTPPLSVLQGKGFRVALVTDGRMSGASGKIPAAIHVSPEAAAGGPL
AKVRDGDLIRLDSVAGTLAVLLPEDEWAARETATLPEAQRIADGRGLGRELFAGMRRNAL
TAEEGACSWL