Protein Info for GFF651 in Variovorax sp. SCN45

Annotation: Phytochrome, two-component sensor histidine kinase (EC 2.7.3.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 762 PF08446: PAS_2" amino acids 32 to 67 (36 residues), 30 bits, see alignment (E = 1.8e-10) PF01590: GAF" amino acids 151 to 312 (162 residues), 52.6 bits, see alignment E=1.8e-17 PF00360: PHY" amino acids 330 to 506 (177 residues), 163.5 bits, see alignment E=8.8e-52 PF00512: HisKA" amino acids 524 to 588 (65 residues), 30.4 bits, see alignment E=8.4e-11 PF02518: HATPase_c" amino acids 633 to 743 (111 residues), 74.6 bits, see alignment E=2.2e-24

Best Hits

KEGG orthology group: None (inferred from 82% identity to vpe:Varpa_3575)

Predicted SEED Role

"Phytochrome, two-component sensor histidine kinase (EC 2.7.3.-)" in subsystem Oxidative stress or Oxygen and light sensor PpaA-PpsR (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.3.-

Use Curated BLAST to search for 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (762 amino acids)

>GFF651 Phytochrome, two-component sensor histidine kinase (EC 2.7.3.-) (Variovorax sp. SCN45)
MSLSNVPSPLISTEPAAGGRPIEPPEVDLSNCDLEPIRVPGSIQPHGRMLVLLPDSLALV
AWSENWPDDQLAAALAAVRGLSLPPLAPGGSPVSLGAMTLAQVTFDAAAHRSNNHLIVEF
EAASPSGGNEAPIYSLGRVFLPRLQEAGTMTELCALAAREMKRLTGFGRCLVYRFDAAGH
GEVLAEEADAGYDSYSGHHFPASDIPRQARALYLVNHFRLIADANYRPVRLRATDAALTA
ADIDLSQAQLRSVSPIHLEYMRNMGTLASMSVSIVIDGKLWGLISCHDHAPRFLDTGTRL
ACEHLGQLLSLQIQSKESNGEVAERLELRQLTLQIVSHLADSDATLQHLTAEVSLMLRVM
RATGAAVVLDDEAWCVGDVPARVHVQELAGWIAGLGVEVYESNALAAHFAPAAQFQDVAA
GVLAISISQVHRHLILWFRPEIVQTVRWAGDPQKPQMVVSGRMHPRLSFASWVEHIRGRS
TPWSVAEVGAAGELRQALIGIVLRRAEELAAVASELGRVNKELEAFSYTVSHDLRAPMRH
IAGYVDLVAQTEGKQLSARAHRYLSHVKDAAAFAGQMVDALLDFSRMGRSALKQRQVDSA
SLVDGLVRELTRLEPQRNIEWQVDADLPLLYADPLLLQVAVRNLLANAVKYSRTRSPSRI
AVRAIHSDLGDGLEVQDNGVGFQMKYVDKLFGVFQRLHQAEDFEGTGIGLANVKRIVERH
GGTVWAKGEIDAGASFGFILPRQGPDNAAASTAPAANGEGHA