Protein Info for GFF650 in Xanthobacter sp. DMC5

Annotation: Glutamate--cysteine ligase EgtA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 469 TIGR01436: glutamate--cysteine ligase" amino acids 23 to 469 (447 residues), 681 bits, see alignment E=2.7e-209 PF04107: GCS2" amino acids 63 to 373 (311 residues), 338.9 bits, see alignment E=1.3e-105

Best Hits

Swiss-Prot: 61% identical to GSH1_TOBAC: Glutamate--cysteine ligase, chloroplastic (GSH1) from Nicotiana tabacum

KEGG orthology group: K01919, glutamate--cysteine ligase [EC: 6.3.2.2] (inferred from 90% identity to xau:Xaut_4204)

MetaCyc: 62% identical to gamma-glutamylcysteine synthetase (Lotus japonicus)
Glutamate--cysteine ligase. [EC: 6.3.2.2]

Predicted SEED Role

"Glutamate--cysteine ligase (EC 6.3.2.2)" in subsystem Glutathione: Biosynthesis and gamma-glutamyl cycle (EC 6.3.2.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (469 amino acids)

>GFF650 Glutamate--cysteine ligase EgtA (Xanthobacter sp. DMC5)
VYGLAPLRLKSFLMARDTLDATPISDRGELVAWMEGGSKPERSFRIGTEHEKIPFTMGRH
EPVPYEGPKGIRKLLEGMRSLLGWEPIMEGETIIGLADVTGGGAISLEPGGQFELSGAPL
RTIHETCSELNAHLAQVREVATPLGIGFLAIGMSPKWSRSETPVMPKGRYKIMANYMPKV
GTLGLDMMFRTCTVQVNLDFSSEADMVKKMRVGLALQPVATALFANSPFTEGKPNGFLSF
RSEIWRDTDNARSGMLPFAFEEGFGFERYVDYALDVPMYFVKRGDHYVDVAGTSFRDLLA
GRHPLLPGETATLSDWINHLSTIFPEVRLKRFLEMRGADAGPSINLCALPAFWVGLLYDQ
QSLDAAWDLVKDWTAEERQTLRDDVPRVALKAEIRGRPLREIAREVLKLSQAGLKRRGFH
DRLGRDETRFLEPLEQAVLTGRAPAEELLELYHGPWNGSVEPAFDALAY