Protein Info for GFF6422 in Variovorax sp. SCN45

Annotation: Threonine dehydratase, catabolic (EC 4.3.1.19) @ L-serine dehydratase, (PLP)-dependent (EC 4.3.1.17)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00291: PALP
  • amino acids 20 to 303 (284 residues), 256 bits, E=7.2e-80
• TIGR01127: threonine ammonia-lyase
  • amino acids 24 to 398 (375 residues), 423.2 bits, E=4.8e-131
• PF13291: ACT_4
  • amino acids 326 to 392 (67 residues), 28.9 bits, E=2.2e-10
• PF01842: ACT
  • amino acids 327 to 361 (35 residues), 27.4 bits, 3.4e-10

Best Hits

KEGG orthology group: K01754, threonine dehydratase [EC: 4.3.1.19] (inferred from 97% identity to vpe:Varpa_0174)

Predicted SEED Role

"Threonine dehydratase (EC 4.3.1.19)" in subsystem Branched-Chain Amino Acid Biosynthesis or Threonine degradation (EC 4.3.1.19)

MetaCyc Pathways

• superpathway of branched chain amino acid biosynthesis (17/17 steps found)
• superpathway of L-isoleucine biosynthesis I (13/13 steps found)
• L-isoleucine biosynthesis I (from threonine) (7/7 steps found)
• superpathway of L-threonine metabolism (14/18 steps found)
• L-methionine degradation II (3/3 steps found)
• L-threonine degradation I (5/6 steps found)
• L-threonine degradation V (1/2 steps found)
• hypoglycin biosynthesis (4/14 steps found)
• cyclosporin A biosynthesis (2/15 steps found)

KEGG Metabolic Maps

• Glycine, serine and threonine metabolism
• Valine, leucine and isoleucine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 4.3.1.19

Use Curated BLAST to search for 4.3.1.19

Protein Sequence (402 amino acids)

>GFF6422 Threonine dehydratase, catabolic (EC 4.3.1.19) @ L-serine dehydratase, (PLP)-dependent (EC 4.3.1.17) (Variovorax sp. SCN45)
MRAMVGIEEIQRAAARLQGQVLNTPCVESRTLSEIVGAQVFLKFENLQFTSSFKERGACN
KLVDLSENGTRGVIAMSAGNHAQGVAYHAQRLGLRALIVMPRFTPGVKIERTRGFGAEVV
LHGDTLDAARAHALELAEREGLTFVHPYDDEAIIAGQGTVALEMLDVVPDLDTLVVAVGG
GGLIAGMTVAARERKPGIEIVGVQTTRFPAMVNAVKGTHHAQGKSTIAEGIAVGTPGVLT
QEIIAKKVDDLLLVDEGDIEQGVLMLLEIEKTLVEGAGAAGLAALIRHPERFKGKRVGLV
LSGGNIDPLLLAAIIERGMVRAGRLARVRVSARDVPGSLAQITATVAEAGANVDEVHHQR
AFTMLAAQNVDIELVLQTRGRAHLASVLTALKDAGFEAEEQH