Protein Info for GFF6409 in Variovorax sp. SCN45
Annotation: Histidine ammonia-lyase (EC 4.3.1.3)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 79% identical to HUTH_ACIAC: Histidine ammonia-lyase (hutH) from Acidovorax citrulli (strain AAC00-1)
KEGG orthology group: K01745, histidine ammonia-lyase [EC: 4.3.1.3] (inferred from 96% identity to vpe:Varpa_0183)MetaCyc: 59% identical to histidase subunit (Pseudomonas putida)
Histidine ammonia-lyase. [EC: 4.3.1.3]
Predicted SEED Role
"Histidine ammonia-lyase (EC 4.3.1.3)" in subsystem Histidine Degradation (EC 4.3.1.3)
MetaCyc Pathways
- L-histidine degradation II (5/5 steps found)
- L-histidine degradation VI (7/8 steps found)
- L-histidine degradation I (3/4 steps found)
- L-histidine degradation III (4/6 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 4.3.1.3
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (519 amino acids)
>GFF6409 Histidine ammonia-lyase (EC 4.3.1.3) (Variovorax sp. SCN45) MPSNHTAKPEATLTLTPGKVDLAMLRHIQAGGVQLALDASVRDGMARAEAAVRHIVENDQ VVYGINTGFGKLASTRIGNDHLAELQRNLVLSHSVGTGEPLTAPVVRMILATKAVSLARG HSGVRPALVDALLALFNAGVTPSIPCKGSVGASGDLAPLAHMACVLIGEGEATLADGKKV SGAEAMRSIGLEPFVLGPKEGLALLNGTQVSTALALAGLFGAEDVFASALMSGALSLEAI QGSIKPFDARIHAARGQPGQIAVAGAVRTLLEGSEIVPSHADCGRVQDPYSVRCIPQVMG ACLDNLAHAARVLVIEANAASDNPLVFTDTGEVISGGNFHAEPVAFAADIIALAVSEVGA IAERRLALLLDSGLSGLPPFLVRDGGLNSGFMIAQVTAAALASENKSLAHPASVDSLPTS ANQEDHVSMATFAARRLGDMVNNTAVVVGIEAMAAAQGIELKRKLKSSPLVEAEFAGIRQ KVAFLETDRYLAPDIEAMRLWALKAELPAALLDILPSHS