Protein Info for GFF64 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Predicted secretion system X protein GspG-like 3

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 143 transmembrane" amino acids 16 to 39 (24 residues), see Phobius details PF07963: N_methyl" amino acids 10 to 34 (25 residues), 38 bits, see alignment 8e-14 TIGR01710: type II secretion system protein G" amino acids 13 to 143 (131 residues), 175.5 bits, see alignment E=5.3e-56 TIGR02532: prepilin-type N-terminal cleavage/methylation domain" amino acids 13 to 34 (22 residues), 31.7 bits, see alignment 9.2e-12 PF08334: T2SSG" amino acids 38 to 143 (106 residues), 140.4 bits, see alignment E=2.2e-45

Best Hits

Swiss-Prot: 42% identical to GSPG_PSEAE: Type II secretion system protein G (xcpT) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K02456, general secretion pathway protein G (inferred from 77% identity to dar:Daro_3221)

Predicted SEED Role

"Predicted secretion system X protein GspG-like 3"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (143 amino acids)

>GFF64 Predicted secretion system X protein GspG-like 3 (Hydrogenophaga sp. GW460-11-11-14-LB1)
MLSLVVPGPRRSRGFTLLELLVVMVIIGLLAGFVGPRFFAQIGKSEVKTARAQIDALGKA
LDQFRLDVGRYPTSEEGLAALNERPGADTRWSGPYLKKGVPPDPWGTPYVYRAPGEHGEY
DLLSLGNDRQPGGSGEAEDIVSW