Protein Info for GFF6393 in Variovorax sp. SCN45

Annotation: 5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13), C-terminal domain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02082: methionine synthase
  • amino acids 2 to 893 (892 residues), 1196.2 bits, E=0
• PF00809: Pterin_bind
  • amino acids 40 to 278 (239 residues), 218.4 bits, E=2.5e-68
• PF02607: B12-binding_2
  • amino acids 342 to 418 (77 residues), 83.8 bits, E=1.5e-27
• PF02310: B12-binding
  • amino acids 440 to 533 (94 residues), 61.4 bits, E=1.5e-20
• PF02965: Met_synt_B12
  • amino acids 633 to 908 (276 residues), 394.7 bits, E=4.5e-122

Best Hits

KEGG orthology group: K00548, 5-methyltetrahydrofolate--homocysteine methyltransferase [EC: 2.1.1.13] (inferred from 86% identity to aaa:Acav_0266)

Predicted SEED Role

"5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13)" in subsystem Methionine Biosynthesis (EC 2.1.1.13)

MetaCyc Pathways

• aspartate superpathway (24/25 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (17/18 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
• folate transformations III (E. coli) (8/9 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (8/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (8/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (7/8 steps found)
• L-methionine biosynthesis III (4/4 steps found)
• folate transformations II (plants) (9/11 steps found)
• L-methionine biosynthesis I (4/5 steps found)
• L-methionine salvage from L-homocysteine (1/3 steps found)
• folate transformations I (8/13 steps found)
• superpathway of L-methionine salvage and degradation (9/16 steps found)

KEGG Metabolic Maps

• Methionine metabolism
• One carbon pool by folate

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.13

Use Curated BLAST to search for 2.1.1.13

Protein Sequence (923 amino acids)

>GFF6393 5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13), C-terminal domain (Variovorax sp. SCN45)
MSQISAPATPDSAPLAPPPMKLSGLEPVAIGEGTLFVNIGERTNVTGSKAFARMILNGQF
EEALAVARQQVENGAQVIDINMDEAMLDSKVAMVRFLNLIASEPDIARVPVMVDSSKWDV
IEAGLRCIQGKGIVNSISMKEGVDKFKHEAKLVKRYGAAAVVMAFDEKGQADTYARKIEI
CERAYRILVDEVGFPPEDIIFDPNIFAIATGIEEHDNYAVDFIEAVRWIKQNLPGAKVSG
GVSNVSFSFRGNDPMREAIHTVFLYHAIQAGMDMGIVNAGMVGVYDDLEPALRERVEDVV
LNRRPDAGERLVEVAETAKSGAKDESKRLEWRGTPEQPVHVNQRLSHAMVHGITDFIVED
TEEAYQQILATGGRPLHVIEGPLMDGMNIVGDLFGAGKMFLPQVVKSARVMKSAVAHLLP
YIEEEKLRDEAAGRDVRTKGKIIIATVKGDVHDIGKNIVTVVLQCNNFEVVNMGVMVPCH
EILARAKVEGADIVGLSGLITPSLEEMQYVAGEMQRDDHFRIKKIPLMIGGATTSRVHTA
VKIAPHYEGPVVYVPDASRSVSVAQSLLSDQATAYIDEINADYEKVRTQHANKKQVPLWP
LAKVRANKTPIDWSNYTPPCPKFIGRRVFRNFDLTELAKYIDWGPFFQTWDLAGPFPAIL
KDEIVGTEAVRVYADGQRMLKRLIEGRWLSASGIVGFWPANTVNDDDIELYTDETRSEVA
LTWYGMRQQTEKQVIDGVMRPSRCLADFVAPKASGLKDYVGMFAVTAGLGVEKKEKYFID
DLDDYSAIMLKALADRLAEAFAESLHHRARTDLWGYASDENLSNDDMIGEKYRGIRPAPG
YPACPDHSVKRPMFDLLGCADIGMTLTESLAMMPAASVSGFYLSHPDSTYFNVGKIGHDQ
LQDQAARRKESEDYLERLLAPNL