Protein Info for GFF639 in Hydrogenophaga sp. GW460-11-11-14-LB1
Annotation: Glutamate-1-semialdehyde aminotransferase (EC 5.4.3.8)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 84% identical to GSA_RHOFT: Glutamate-1-semialdehyde 2,1-aminomutase (hemL) from Rhodoferax ferrireducens (strain ATCC BAA-621 / DSM 15236 / T118)
KEGG orthology group: K01845, glutamate-1-semialdehyde 2,1-aminomutase [EC: 5.4.3.8] (inferred from 84% identity to rfr:Rfer_1369)MetaCyc: 56% identical to glutamate-1-semialdehyde 2,1-aminomutase (Escherichia coli K-12 substr. MG1655)
Glutamate-1-semialdehyde 2,1-aminomutase. [EC: 5.4.3.8]
Predicted SEED Role
"Glutamate-1-semialdehyde aminotransferase (EC 5.4.3.8)" in subsystem Experimental tye or Heme and Siroheme Biosynthesis (EC 5.4.3.8)
MetaCyc Pathways
- adenosylcobalamin biosynthesis II (aerobic) (28/33 steps found)
- adenosylcobalamin biosynthesis I (anaerobic) (29/36 steps found)
- superpathway of heme b biosynthesis from glutamate (9/10 steps found)
- tetrapyrrole biosynthesis I (from glutamate) (6/6 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 5.4.3.8
Use Curated BLAST to search for 5.4.3.8
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (434 amino acids)
>GFF639 Glutamate-1-semialdehyde aminotransferase (EC 5.4.3.8) (Hydrogenophaga sp. GW460-11-11-14-LB1) MTTDRNQQLFDRAKALIPGGVNSPVRAFRAVGGTPRFVQRAQGAHFWDANGQRYIDYIGS WGPMILGHGHPAVVEAVQKAVLEGFSFGAPTEREVELAEAIVELVPSIDMVRLVSSGTEA GMSTIRLARGATGRKKIIKFEGCYHGHADALLVKAGSGLATFGNPTSSGVPPEVVQHTLV LEYNNLEQLEAAFAEHGPEVACLIIEPIAGNMNFVRASVPFMKRCRELCTQHGALLAFDE VMTGFRVALGGAQSVYAQLIPGFEPDMTVMGKVIGGGMPLAAFGAKRAVMEQLAPLGGVY QAGTLSGNPVATACGLATLKEIRKPGFYDALAATTRSLVDGLKGAAAAEGVALSGDSEGG MFGFFLFDELPQNYAKVMTTDGPRFNTLFHGLLERGVYIAPALYEAGFVSAAHTAKDIED TVAAAREVFKQLAR