Protein Info for GFF6333 in Variovorax sp. SCN45
Annotation: 1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 63% identical to GLGB_KLEP7: 1,4-alpha-glucan branching enzyme GlgB (glgB) from Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)
KEGG orthology group: K00700, 1,4-alpha-glucan branching enzyme [EC: 2.4.1.18] (inferred from 91% identity to vpe:Varpa_0443)MetaCyc: 62% identical to 1,4-alpha-glucan branching enzyme (Escherichia coli K-12 substr. MG1655)
1,4-alpha-glucan branching enzyme. [EC: 2.4.1.18]
Predicted SEED Role
"1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18)" in subsystem Glycogen metabolism or Trehalose Biosynthesis (EC 2.4.1.18)
MetaCyc Pathways
- glycogen biosynthesis I (from ADP-D-Glucose) (4/4 steps found)
- starch biosynthesis (6/10 steps found)
- glycogen biosynthesis III (from α-maltose 1-phosphate) (4/8 steps found)
- glycogen biosynthesis II (from UDP-D-Glucose) (1/4 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 2.4.1.18
Use Curated BLAST to search for 2.4.1.18
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (724 amino acids)
>GFF6333 1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18) (Variovorax sp. SCN45) MPLKPLSESEIRALMRAEHGDPFAVLGPHETAEGLEVRALLPGAQRVAVVHSRTGWPLSI LSRLGESDIFSGLVPAAEARMGYSFQIDWDAHTSRLEDPYRFPFVLGDTDVWLLAEGTHL RPWERLGAHLREMNGVKGVAFAVWAPNARRVSVVGNFNNWDGRRHMMRLRRECGVWEIFA PHVAVGDAYEFEILSAHGEVLRKADPFAFSAQLRPDTACVVQPLPAAVKMPEGRAEANGR HAPVSIYEVHLGSWRRKNNGHEWLDYRELADTLVPYARDMGFTHIELLPISEHPFDGSWG YQPIGLYAPTSRFGTPGDFRHFVEAAHAAGLGVILDWVPAHFPTDAHGLGRFDGTALYEY ADPREGFHNDWQTLIFNYARTEVRNYLVGNALYWLERYGVDGLRVDAVASMLYRDYSRKP GEWVPNSLGGRENLEAIDFLRRMNRVVGVERPGAVTLAEESTSFPGVTRPPEDGGLGFHY KWNMGWMNDTLAYAGTDPVYRKHHHQKITFGLMYAHSENFVLPLSHDEVVHGKGSLIGRM PGDEWQKFAGLRNLYGYLWAYPGKKLLFMGGEFAQYAEWNADRSLDWHLLEHAAHQGVRR LVRDLNNVYRHFPALHELDHDGDGFEWIVHDDSDQSVFAFVRRSRDGAFVVAVCNFTPVP RHGYRLGVPCAGSYREIINTDGVVYGGSGVGNGVVESSAVAWHGKADSLSVSLPPLATLM WVLV