Protein Info for GFF6323 in Variovorax sp. SCN45

Annotation: Benzoate transport protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 425 signal peptide" amino acids 1 to 37 (37 residues), see Phobius details transmembrane" amino acids 46 to 65 (20 residues), see Phobius details amino acids 71 to 89 (19 residues), see Phobius details amino acids 95 to 114 (20 residues), see Phobius details amino acids 126 to 150 (25 residues), see Phobius details amino acids 166 to 185 (20 residues), see Phobius details amino acids 197 to 222 (26 residues), see Phobius details amino acids 250 to 278 (29 residues), see Phobius details amino acids 290 to 315 (26 residues), see Phobius details amino acids 321 to 342 (22 residues), see Phobius details amino acids 352 to 381 (30 residues), see Phobius details PF03594: BenE" amino acids 6 to 382 (377 residues), 446.2 bits, see alignment E=4.5e-138 TIGR00843: benzoate transporter" amino acids 10 to 382 (373 residues), 395.7 bits, see alignment E=1.1e-122

Best Hits

Swiss-Prot: 49% identical to YDCO_ECOLI: Inner membrane protein YdcO (ydcO) from Escherichia coli (strain K12)

KEGG orthology group: K05782, benzoate membrane transport protein (inferred from 86% identity to vap:Vapar_0431)

Predicted SEED Role

"Benzoate transport protein" in subsystem Benzoate degradation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (425 amino acids)

>GFF6323 Benzoate transport protein (Variovorax sp. SCN45)
MSFFKDLSLSAFTAGFVAVLVGFTSSVAIVFQAAQAFGATPELAASWMWALGIGMGLASA
LPSLWWKKPVMIAWSTPGAAVLAVAGMGYGMGEAVGAFIVCAVLIVLAGATGWFERVMNR
IPMAIASALLAGVLARFGLSAFIAAQTALPLVLLMLGTYLVGKRLLPRYAVPLTLLVAIV
YAAARGQLAWSAVHFSFTWPVFTMPVFSWQAIVSLALPLFVVTMASQNLPGVAAIRASGY
GDLPVSKLITISGLTTLVLAPFGAFALNLSAITAAICMGREAHEDPARRYTAAVSCGAIY
VVIGLFGAAVTGLLTAFPKELVAAIAGLALLGTIGGGLAAAVRDESHREAALITFLVTLS
GVTLAGIGSAFWGVVAGAVALAVQHVGRASPPEKAPAGKDIAASGNIAPDTQVQVAAAPG
AGKNA