Protein Info for GFF6305 in Variovorax sp. SCN45

Annotation: Malate synthase G (EC 2.3.3.9)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01345: malate synthase G
  • amino acids 5 to 722 (718 residues), 1187.9 bits, E=0
• PF20656: MS_N
  • amino acids 18 to 76 (59 residues), 44.8 bits, 1.5e-15
• PF20658: MSG_insertion
  • amino acids 160 to 234 (75 residues), 115.3 bits, E=2.4e-37
• PF01274: MS_TIM-barrel
  • amino acids 336 to 571 (236 residues), 220 bits, E=5.2e-69
• PF20659: MS_C
  • amino acids 592 to 689 (98 residues), 90.9 bits, E=1.7e-29

Best Hits

Swiss-Prot: 96% identical to MASZ_VARPS: Malate synthase G (glcB) from Variovorax paradoxus (strain S110)

KEGG orthology group: K01638, malate synthase [EC: 2.3.3.9] (inferred from 98% identity to vpe:Varpa_0471)

MetaCyc: 62% identical to malate synthase G (Escherichia coli K-12 substr. MG1655)
Malate synthase. [EC: 2.3.3.9]

Predicted SEED Role

"Malate synthase G (EC 2.3.3.9)" in subsystem Photorespiration (oxidative C2 cycle) (EC 2.3.3.9)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (24/26 steps found)
• superpathway of glyoxylate bypass and TCA (11/12 steps found)
• glyoxylate cycle (6/6 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• glycolate and glyoxylate degradation II (2/2 steps found)
• superpathway of glycol metabolism and degradation (5/7 steps found)
• L-arabinose degradation IV (5/8 steps found)
• chitin deacetylation (2/4 steps found)
• D-xylose degradation IV (3/7 steps found)
• crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered) (3/14 steps found)
• superpathway of pentose and pentitol degradation (16/42 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.3.9

Protein Sequence (724 amino acids)

>GFF6305 Malate synthase G (EC 2.3.3.9) (Variovorax sp. SCN45)
MTARTTAHGLQVATELHRFIEDKVLPASGVASDVFWKGFDAIVSDLAPKNIALLAERDRL
QSELDAWHKKNPGPIADMPAYRAFLEKIGYLLPQPQGAKATTANVDSELALQAGPQLVVP
ILNARYALNAANARWGSLYDALYGTDAIPETGGAEKGKGYNPARGAKVIEFARNVLDQAA
PLANGSHKQATGYSVKDGKLVVALQSSSTGLADASQFIGYQGDAAAPSSVLLKHNGIHLD
IRIDRSTPIGKSDAAGVSDLVLEAALSTILDLEDSVAVVDAADKVVAYSNWLGIVEGTLT
EEVAKGGKTFTRGLNPDREYTGADGQPVKLHGRSLMFLRNVGHLMTNPAILYAGGKEIPE
GILDAVVTTTIATIDLKRKGNSRTGSIYIVKPKMHGPAEVAFASELFGRVEQLLGLPANT
VKLGIMDEERRTSVNLKACIAEAAARVAFINTGFLDRTGDEMHTAMQGGPMIRKGDMKSS
AWIGAYEKNNVLVGLSCGLRGKAQIGKGMWAMPDLMAAMLEQKIAHPKAGANTAWVPSPT
AATLHALHYHQVSVTAVQQELEKIDADAERDNILNALLQVPIAAEAKWTAEERQQEIDNN
VQGILGYVVRWIDQGVGCSKVPDIHNVGLMEDRATLRISSQHIANWLLHGVVTKAQVDET
FQRMAKVVDEQNAGDALYQPMAGNFATSAAYKAAQDLVFKGIEQPSGYTEPLLHAWRLKV
KGEA