Protein Info for GFF6301 in Variovorax sp. SCN45
Annotation: Pyruvate:Oxaloacetate transcarboxylase domain protein
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 39% identical to LIUE_PSEAE: 3-hydroxy-3-isohexenylglutaryl-CoA/hydroxy-methylglutaryl-CoA lyase (liuE) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
KEGG orthology group: K01640, hydroxymethylglutaryl-CoA lyase [EC: 4.1.3.4] (inferred from 95% identity to vpe:Varpa_0474)MetaCyc: 39% identical to hydroxymethylglutaryl-CoA lyase subunit (Pseudomonas aeruginosa PAO1)
Hydroxymethylglutaryl-CoA lyase. [EC: 4.1.3.4]; 3-hydroxy-3-isohexenylglutaryl-CoA lyase. [EC: 4.1.3.4, 4.1.3.26]
Predicted SEED Role
"Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4)" in subsystem HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism (EC 4.1.3.4)
MetaCyc Pathways
- ketogenesis (5/5 steps found)
- L-leucine degradation I (5/6 steps found)
- mevalonate degradation (2/2 steps found)
- cis-geranyl-CoA degradation (3/9 steps found)
KEGG Metabolic Maps
- Butanoate metabolism
- Geraniol degradation
- Synthesis and degradation of ketone bodies
- Valine, leucine and isoleucine degradation
Isozymes
Compare fitness of predicted isozymes for: 4.1.3.4
Use Curated BLAST to search for 4.1.3.26 or 4.1.3.4
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (312 amino acids)
>GFF6301 Pyruvate:Oxaloacetate transcarboxylase domain protein (Variovorax sp. SCN45) MTPPDVLISEVGPRDGLQSVKATMPTDDKLRWIDALYAAGVREIEVASFVPAKLLPQMAD AAEVVRHAVTLPGLVVMALVPNRKGAQAALEAGVHKLTMPVSASVAHSLANVRKTPTEMV EEVRAIAELRNAIAPRVKLEAGISTAFGCTLQGLVPEDDVIRLAAQCVEAGADESGLSDT VGYANPAQVRRLFRRLRAEIGVHAGAAHMHNTRGLGIANCLAAWDEGVRTFDASLGGLGG CPYAPGASGNAVTEDLVFMFEAMGVRTGIDIQKLIAARAPLMAGLPGEPVYGMTPEAGLP KGFVQEHIEQHV