Protein Info for GFF623 in Xanthobacter sp. DMC5

Annotation: Ribonuclease 3

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 251 TIGR02191: ribonuclease III" amino acids 26 to 240 (215 residues), 216.8 bits, see alignment E=1.3e-68 PF14622: Ribonucleas_3_3" amino acids 36 to 162 (127 residues), 98.4 bits, see alignment E=5.6e-32 PF00636: Ribonuclease_3" amino acids 61 to 151 (91 residues), 66.6 bits, see alignment E=4.5e-22 PF00035: dsrm" amino acids 177 to 240 (64 residues), 51.9 bits, see alignment E=1.4e-17

Best Hits

Swiss-Prot: 81% identical to RNC_AZOC5: Ribonuclease 3 (rnc) from Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / NBRC 14845 / NCIMB 13405 / ORS 571)

KEGG orthology group: K03685, ribonuclease III [EC: 3.1.26.3] (inferred from 93% identity to xau:Xaut_3881)

Predicted SEED Role

"Ribonuclease III (EC 3.1.26.3)" in subsystem RNA processing and degradation, bacterial or Two cell division clusters relating to chromosome partitioning (EC 3.1.26.3)

Isozymes

Compare fitness of predicted isozymes for: 3.1.26.3

Use Curated BLAST to search for 3.1.26.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (251 amino acids)

>GFF623 Ribonuclease 3 (Xanthobacter sp. DMC5)
MALDGAMGSAVHTGTLNGAGRDLAHLEERIGYRFRDRSILELALSHISAVKGEAPRLRSY
QRVEFLGDHVLGSVVSHMLYLAFPEGEEGELSRRLAELVREEACAEVAEDMDLGPFIRLG
PGESQSGAHKRRAILADVAEGVVAAVFLDGGYEAAHALVERFWRPRLEAPRRPLRDAKTV
LQEWAQARGLPPPVYREVMRSGPDHAPRFTVAVDLPGLVSAQADGASKQNAQKAAAAAFL
VREGVWEKDEA