Protein Info for GFF622 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Phenylacetate-coenzyme A ligase (EC 6.2.1.30)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02155: phenylacetate-CoA ligase
  • amino acids 10 to 429 (420 residues), 712.8 bits, E=5.4e-219
• PF00501: AMP-binding
  • amino acids 81 to 288 (208 residues), 72.5 bits, E=2.9e-24
• PF14535: AMP-binding_C_2
  • amino acids 334 to 429 (96 residues), 85.3 bits, E=2.9e-28

Best Hits

Swiss-Prot: 72% identical to PAAK_AZOEV: Phenylacetate-coenzyme A ligase (paaK) from Azoarcus evansii

KEGG orthology group: K01912, phenylacetate-CoA ligase [EC: 6.2.1.30] (inferred from 78% identity to bmu:Bmul_2855)

MetaCyc: 72% identical to phenylacetate-CoA ligase (aerobic) (Aromatoleum evansii)
Phenylacetate--CoA ligase. [EC: 6.2.1.30]

Predicted SEED Role

"Phenylacetate-coenzyme A ligase (EC 6.2.1.30)" in subsystem Aromatic amino acid interconversions with aryl acids (EC 6.2.1.30)

MetaCyc Pathways

• phenylacetate degradation I (aerobic) (8/9 steps found)
• superpathway of phenylethylamine degradation (9/11 steps found)
• penicillin G and penicillin V biosynthesis (1/4 steps found)
• phenylacetate degradation II (anaerobic) (1/4 steps found)
• L-phenylalanine degradation IV (mammalian, via side chain) (4/9 steps found)
• anaerobic aromatic compound degradation (Thauera aromatica) (6/27 steps found)

KEGG Metabolic Maps

• Phenylalanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.30

Use Curated BLAST to search for 6.2.1.30

Protein Sequence (431 amino acids)

>GFF622 Phenylacetate-coenzyme A ligase (EC 6.2.1.30) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MKTFPLEPIEKAGIDELRALQLKRLQATLRHAYQNSPVYRAKFDAAGVHPDDCRSLADLA
KFPFTTKKDLRDSYPFGMFAVPREQCARIHASSGTTGKPTVVGYTLKDIDTWATVMARSI
RASGARPGDLVHVSYGYGLFTGGLGAHYGAEKLGLTVVPFGGGQTERQVQLIQDFRPDII
MVTPSYMLAIADEFERQGVDPRESSLRLGIFGAEPWTNDMRLAIEQRMGIDAVDIYGLSE
VMGPGVANECVETKDGPTIWEDHFYPEIIDPDTGEPVPDGEMGELVFTSLTKEALPIIRY
RTRDLTRLLPGTARPMRRMEKITGRSDDMMIVRGVNVFPTQIEELILKRAELSAHYQCVL
TRQGPMDDLTVVVETRSGLSPESEEAQGAARLLQHDIKVFVGSSVMVQLKPEGGVERSQG
KAKRVLDQRGR