Protein Info for GFF620 in Xanthobacter sp. DMC5

Annotation: Pyridoxine 5'-phosphate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 256 PF03740: PdxJ" amino acids 8 to 244 (237 residues), 315.4 bits, see alignment E=8.8e-99 TIGR00559: pyridoxine 5'-phosphate synthase" amino acids 8 to 245 (238 residues), 280.3 bits, see alignment E=6.7e-88

Best Hits

Swiss-Prot: 87% identical to PDXJ_XANP2: Pyridoxine 5'-phosphate synthase (pdxJ) from Xanthobacter autotrophicus (strain ATCC BAA-1158 / Py2)

KEGG orthology group: K03474, pyridoxine 5-phosphate synthase [EC: 2.6.99.2] (inferred from 87% identity to xau:Xaut_3878)

MetaCyc: 54% identical to pyridoxine 5'-phosphate synthase (Escherichia coli K-12 substr. MG1655)
Pyridoxine 5'-phosphate synthase. [EC: 2.6.99.2]

Predicted SEED Role

"Pyridoxine 5'-phosphate synthase (EC 2.6.99.2)" in subsystem Pyridoxin (Vitamin B6) Biosynthesis (EC 2.6.99.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.6.99.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (256 amino acids)

>GFF620 Pyridoxine 5'-phosphate synthase (Xanthobacter sp. DMC5)
MAVLKPVRLGVNVDHVATVRNARGGLLPDPVRAALLAAAAGADGITAHLREDRRHIRDAD
MTRLKAEIDLPLNFEMAATEEMVGIAVSVRPNACCLVPERREERTTEGGLDAAGLAAELG
PRIARLKAAGIRVSLFIAAEERQIAEAATLGADIVELHTGGWCDAVAEGRTAEAEAEFAR
LKAGARQANALGLEVHAGHGLDYATAERIAAFPEIVELNIGHFLIGEAIFVGLEAAIRTM
RAAIAAGRAAAAGEAA