Protein Info for GFF619 in Variovorax sp. SCN45

Annotation: Aspartate carbamoyltransferase (EC 2.1.3.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00670: aspartate carbamoyltransferase
  • amino acids 91 to 400 (310 residues), 305.4 bits, E=2e-95
• PF02729: OTCace_N
  • amino acids 91 to 231 (141 residues), 153.9 bits, E=3.3e-49
• PF00185: OTCace
  • amino acids 243 to 398 (156 residues), 93.7 bits, E=1.2e-30

Best Hits

KEGG orthology group: K00609, aspartate carbamoyltransferase catalytic subunit [EC: 2.1.3.2] (inferred from 75% identity to bph:Bphy_4730)

Predicted SEED Role

"Aspartate carbamoyltransferase (EC 2.1.3.2)" in subsystem De Novo Pyrimidine Synthesis (EC 2.1.3.2)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (43/46 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (17/18 steps found)
• UMP biosynthesis II (6/6 steps found)
• UMP biosynthesis III (6/6 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (8/9 steps found)
• UMP biosynthesis I (5/6 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.1.3.2

Use Curated BLAST to search for 2.1.3.2

Protein Sequence (432 amino acids)

>GFF619 Aspartate carbamoyltransferase (EC 2.1.3.2) (Variovorax sp. SCN45)
MNNMPQQVLLRDAMRQLNMTRDAFAERIGSRRRALDAWLLPDESNEFRAMPDVARRFVSE
ILASDGLRGEYTQSAHEGALRKAMATGGAHHLLSVNQLNRDAIDELFQLADVMQPIARRR
KVSRVLEGAVLANLFFEASTRTRVSFGAAFCRLGGSVCDTTGFTFSSMAKGESIYDTSRV
IGGYVDAMVVRHPEQGSVAEFARATNVPVINGGDGPGEHPSQALLDLYTIQREFSRLGKL
VDGAHIAMVGDLKYGRTVHSLIKLLALYKGLKFTLISPPSLAMPAAIVEAVSRNGHVVET
AATPALGLAGADIIYATRIQKERFANDEVLEGFDNAFEINQALIESACKQDVVVMHPLPR
DGRPGSHDLSTDLNNDPRLAIFRQTDNGIPVRMAIFATLLGVDKLVSRSMRDAAWTPPSH
IGPDDSVFHGLD