Protein Info for GFF6185 in Variovorax sp. SCN45

Annotation: DNA gyrase subunit A (EC 5.99.1.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 881 TIGR01063: DNA gyrase, A subunit" amino acids 10 to 856 (847 residues), 1176.8 bits, see alignment E=0 PF00521: DNA_topoisoIV" amino acids 32 to 514 (483 residues), 562.5 bits, see alignment E=6.4e-173 PF03989: DNA_gyraseA_C" amino acids 550 to 596 (47 residues), 38.5 bits, see alignment 6.2e-14 amino acids 600 to 643 (44 residues), 35.7 bits, see alignment 4.6e-13 amino acids 660 to 704 (45 residues), 35 bits, see alignment 7.8e-13 amino acids 709 to 754 (46 residues), 44.6 bits, see alignment 7.6e-16 amino acids 760 to 806 (47 residues), 41.3 bits, see alignment 8.6e-15 amino acids 810 to 855 (46 residues), 45.5 bits, see alignment 4.2e-16

Best Hits

KEGG orthology group: K02469, DNA gyrase subunit A [EC: 5.99.1.3] (inferred from 90% identity to aav:Aave_3280)

Predicted SEED Role

"DNA gyrase subunit A (EC 5.99.1.3)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.3)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.3

Use Curated BLAST to search for 5.99.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (881 amino acids)

>GFF6185 DNA gyrase subunit A (EC 5.99.1.3) (Variovorax sp. SCN45)
MTSFAKETLPISLEEEMRSSYLDYAMSVIVGRALPDARDGLKPVHRRVLFAMHELNNDWN
RPYKKSARIVGDVIGKYHPHGDQSVYDTIVRLAQDFSMRHMLVDGQGNFGSVDGDNAAAM
RYTEIRLAKIAHEMLADIDKETVDFQDNYDGSEKEPKVLPSQLPNLLVNGSGGIAVGMAT
NIPPHNLNEVVDACLYLLRNPEASIDELMEIVPAPDFPTAGIIYGINGVKDGYRTGRGKV
VMRAKCHFEDIDRGQRQAIIVDELPYQVNKKTLQERMAELVHEKKIEGISHIQDESDKSG
MRLVIELKRGEVPEVVLNNLYKQTQLQDTFGINMVALVDGQPKLCNLKDLIQVFLQHRRE
VVTRRTVFNLRKARERGHVLEGLAVALANIDEFIRIIRESPTPPVAKAELMTRSWDSKLV
REMLTRSRADGGVINADDYRPEGLEREYGMGGDGLYRLSDTQAQEILQMRLQRLTGLEQD
KIVAEYKDVMAEIDDLLDILAKPERVSTIIGEELGTIKQEFGQSKIGARRSLVEHSAYDL
STEDLITPTDMVVTLSHSGYIKSQPLGEYRAQKRGGRGKQATATKDDDWIDQLFIANTHD
YILCFSNRGRLYWLKVWEVPAGSRGSRGRPIVNMFPLQEGEKINVALALTGEKRNFPADQ
YVFMATSMGTVKKTALDEFNNPRKGGIIAVNLDEGDYLIGAALTDGKHDVMLFSDGGKAV
RFDEEDVRPLGRNARGVRGMSLEAGQGVIAMLVAEDEQQSVLTATENGYGKRTSITEYTR
HGRGTKGMIAIQQSERNGKVVAATLVHSDDEIMLITDKGVLVRTRVAEIRELGRATQGVT
LIGLDEGAKLSGLQRIVENDANGETEPDADDTSSSSTENPQ