Protein Info for GFF6125 in Variovorax sp. SCN45

Annotation: Thiosulfate sulfurtransferase, rhodanese (EC 2.8.1.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00581: Rhodanese
  • amino acids 8 to 147 (140 residues), 48 bits, E=7.2e-17
  • amino acids 176 to 290 (115 residues), 37.5 bits, E=1.4e-13

Best Hits

KEGG orthology group: K01011, thiosulfate/3-mercaptopyruvate sulfurtransferase [EC: 2.8.1.1 2.8.1.2] (inferred from 92% identity to vap:Vapar_1566)

Predicted SEED Role

"Thiosulfate sulfurtransferase, rhodanese (EC 2.8.1.1)" (EC 2.8.1.1)

MetaCyc Pathways

• thiosulfate disproportionation IV (rhodanese) (1/1 steps found)
• L-cysteine degradation III (1/2 steps found)
• sulfide oxidation IV (mitochondria) (2/5 steps found)

KEGG Metabolic Maps

• Cysteine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.8.1.1 or 2.8.1.2

Protein Sequence (302 amino acids)

>GFF6125 Thiosulfate sulfurtransferase, rhodanese (EC 2.8.1.1) (Variovorax sp. SCN45)
MYTTLVSVEQLRQLQAGDKPFMLFDCSFELMKPEAGAQQYAAAHIPGALYANLDTDLSAK
HGAPGAHGEVVTAQEDGVPASGGRHPLPSREKFAAWLSSIGFANDMQAVVYDRNGANYCG
RLWWMLKWMGHDAVAVLDGGLQAWQAAGGEVTDREEPARFQSNFVTGEPIAKLVTTDTVV
RRLGQPDQNLIDARAGARYRGEVEPLDPIAGHIPGALNRPFAENIGPDGKFKPASQLRAE
FEALLAGRDAATVVHQCGSGVSAVPNLLAMQIAGFGTTALYAGSWSEWSNTPGLPTRQGA
EP