Protein Info for GFF6110 in Variovorax sp. SCN45

Annotation: N-acyl-D-amino-acid deacylase (EC 3.5.1.81)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 487 PF07969: Amidohydro_3" amino acids 50 to 222 (173 residues), 59.7 bits, see alignment E=3.9e-20 amino acids 335 to 464 (130 residues), 50 bits, see alignment E=3.3e-17 PF01979: Amidohydro_1" amino acids 58 to 461 (404 residues), 35 bits, see alignment E=1e-12

Best Hits

Swiss-Prot: 60% identical to NDAD_ALCXX: D-aminoacylase (dan) from Alcaligenes xylosoxydans xylosoxydans

KEGG orthology group: K06015, N-acyl-D-amino-acid deacylase [EC: 3.5.1.81] (inferred from 93% identity to vpe:Varpa_1698)

Predicted SEED Role

"D-aminoacylase (EC 3.5.1.81)" (EC 3.5.1.81)

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.81

Use Curated BLAST to search for 3.5.1.81

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (487 amino acids)

>GFF6110 N-acyl-D-amino-acid deacylase (EC 3.5.1.81) (Variovorax sp. SCN45)
MTEPIPHYDLLIRGGTVIDGTKAPRFNADVGITAGRIAAIGKLAGHTAEQTIDATGRIVA
PGFIDSHTHDDQAVLSQAQMPFKVSQGVTTVVAGNCGISAAPLRIDMDLPMPLSLLESPA
EGRFTSFAAYLDALRATPSSVNVAAMVGHSTLRAVVMADLDRPANDSEIAAMQALVEEAM
QAGAIGMSTGTFYPPAVKATTEEIIEVGRPLTARKALYVTHMRDESDRVMESLDETFQIG
RALDIPVVVSHHKVQNTQNFGKTKVTLPFIKEAMQHQCIGLDCYPYTAGSTMIRTDRGMM
EGRVLIASSVPHPECAGRDLKDIAAEWGVSGEEAAKRLQPGSAIYFMMDEDDVQRILAFD
DTMIGSDGIPLGDKPHPRLWGTFPRVLGHYSRDIGLFPLETAVWKMTGLTARNFGLHERG
TLKPGHHADVVIFNAATVRDTANYETPMQPAEGIDAVIVNGAVTWRDGVHSGARNGQVIT
RRDTAAA