Protein Info for GFF61 in Xanthobacter sp. DMC5

Annotation: Ubiquinol-cytochrome c reductase iron-sulfur subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 210 transmembrane" amino acids 50 to 71 (22 residues), see Phobius details PF10399: UCR_Fe-S_N" amino acids 38 to 77 (40 residues), 76.6 bits, see alignment 7.8e-26 TIGR01409: Tat (twin-arginine translocation) pathway signal sequence" amino acids 45 to 72 (28 residues), 21 bits, see alignment (E = 3.1e-08) TIGR01416: ubiquinol-cytochrome c reductase, iron-sulfur subunit" amino acids 46 to 210 (165 residues), 238.2 bits, see alignment E=5.8e-75 PF00355: Rieske" amino acids 140 to 193 (54 residues), 36.8 bits, see alignment E=3e-13

Best Hits

Swiss-Prot: 66% identical to UCRI_BRADU: Ubiquinol-cytochrome c reductase iron-sulfur subunit (petA) from Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)

KEGG orthology group: K00411, ubiquinol-cytochrome c reductase iron-sulfur subunit [EC: 1.10.2.2] (inferred from 93% identity to xau:Xaut_2957)

Predicted SEED Role

"Ubiquinol-cytochrome C reductase iron-sulfur subunit (EC 1.10.2.2)" in subsystem Ubiquinone Menaquinone-cytochrome c reductase complexes (EC 1.10.2.2)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.10.2.2

Use Curated BLAST to search for 1.10.2.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (210 amino acids)

>GFF61 Ubiquinol-cytochrome c reductase iron-sulfur subunit (Xanthobacter sp. DMC5)
MSMRLPCCVRPVAGGFRGRRLRDVHRDDNIIGDCAVAHTETSDTTTRRDFLYIATGAVGA
VGAAAMAWPFISQLQPDASVLALSTTEVDLSPIAEGQIVTVQWRGKPIFISHRTPAEIKS
AVDTPLSDLKDPQPDSARVKTGKEQWLVVIGICTHLGCVPLGHQGQYNGWFCPCHGSVYD
TSGRIRQGPAPLNLELPPYAFTTDTKIVIG