Protein Info for GFF6097 in Variovorax sp. SCN45

Annotation: 1,2-phenylacetyl-CoA epoxidase, subunit E (EC 1.14.13.149)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 361 TIGR02160: phenylacetate-CoA oxygenase/reductase, PaaK subunit" amino acids 5 to 359 (355 residues), 458.6 bits, see alignment E=5.8e-142 PF00970: FAD_binding_6" amino acids 19 to 104 (86 residues), 48.2 bits, see alignment E=2.2e-16 PF00175: NAD_binding_1" amino acids 119 to 225 (107 residues), 65.3 bits, see alignment E=1.5e-21 PF00111: Fer2" amino acids 277 to 349 (73 residues), 64.4 bits, see alignment E=1.5e-21

Best Hits

KEGG orthology group: K02613, phenylacetic acid degradation NADH oxidoreductase (inferred from 94% identity to vpe:Varpa_1324)

Predicted SEED Role

"Phenylacetate-CoA oxygenase/reductase, PaaK subunit"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (361 amino acids)

>GFF6097 1,2-phenylacetyl-CoA epoxidase, subunit E (EC 1.14.13.149) (Variovorax sp. SCN45)
MSTLFHPLRVKAIEPDTAEAVIVSFEVPTDLQEVFGFTQGQYLTLRKDIDGQDLRRSYSI
CAGLDDGELRVGVRKVRGGVFSNWINANLHPGDTLQVMAPQGRFFVPIEPSSARHHVGIA
GGSGITPILSIMKTVLAREPRSRFTLIYGNRQLQSTMFKEEIEDLKNRYMTRLVLQHVFS
DEHTDSPLGFGVMNREKIGEFLQGVVPAAQIDHVYVCGPFQMNDEAEAALLAAGVPEDRI
HIERFGVALPSATQVGAVVHEAQPGDAKQSRITIVRDGLQREITFTEGQPSILDAASAAG
LEVPFSCTSGVCGTCRAKCVDGEVRMERNFALDKNEVAAGFVLTCQAHPLTESVTLSFDE
R