Protein Info for GFF6054 in Variovorax sp. SCN45

Annotation: Isoleucyl-tRNA synthetase (EC 6.1.1.5)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 949 TIGR00392: isoleucine--tRNA ligase" amino acids 21 to 866 (846 residues), 844.1 bits, see alignment E=6.8e-258 PF00133: tRNA-synt_1" amino acids 34 to 666 (633 residues), 429.1 bits, see alignment E=4.2e-132 PF09334: tRNA-synt_1g" amino acids 64 to 204 (141 residues), 45.7 bits, see alignment E=1.1e-15 PF08264: Anticodon_1" amino acids 712 to 864 (153 residues), 91.3 bits, see alignment E=1.4e-29 PF06827: zf-FPG_IleRS" amino acids 910 to 937 (28 residues), 35.6 bits, see alignment (E = 1.6e-12)

Best Hits

Swiss-Prot: 60% identical to SYI_BORPD: Isoleucine--tRNA ligase (ileS) from Bordetella petrii (strain ATCC BAA-461 / DSM 12804 / CCUG 43448)

KEGG orthology group: K01870, isoleucyl-tRNA synthetase [EC: 6.1.1.5] (inferred from 78% identity to ajs:Ajs_3399)

Predicted SEED Role

"Isoleucyl-tRNA synthetase (EC 6.1.1.5)" (EC 6.1.1.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.5

Use Curated BLAST to search for 6.1.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (949 amino acids)

>GFF6054 Isoleucyl-tRNA synthetase (EC 6.1.1.5) (Variovorax sp. SCN45)
MSDAASTKDYRSTLNLPDTPFPMRGDLPKREPGWVKEWNDEGRYHRLRDARHGAPKFILH
DGPPYANGQIHMGHAVNKILKDMITKARQLEGYDALYVPGWDCHGLPIENAIEKQFGRNL
SRDEMQAKSRAFATGQIAQQMADFQRLGVLGEWDHPYKTMDFANEAGELRAFKRVIERGF
VYRGLKPVYWCFDCGSSLAEFEIEYADKKSQTIDVAFKAHEREKVLKAFGTDHTILGDIF
AVIWTTTAWTIPANQAINLNPELEYSLVDTERGLLILANSLVEMCMTRYALDGKVLATVK
GEKLGGLEFEHPLYDVDAGYKRLSPIYLADYATATDGTGLVHSSPAYGVDDFNSCIAHGV
AYDDILNPVQGNGSYAPDFPLFGGQNIWKAVPVIVAALRDANRLLTTETITHSYPHCWRH
KTPVIYRAAAQWFVRMDEGEGVFTKDKAPKTLRQTALDAIEQTSFYPENGKARLHDMIAG
RPDWCISRQRSWGVPIPFFLHKDSGELHPRTMEILDQAADIVEKGGIEAWSRVTVEEILG
AEDAPHYTKSTDILEVWFDSGSTFSHVLRGTHPTVHHDTGPEADLYLEGHDQHRGWFHSS
LLIACALEDRAPYRGLLTHGFTVDAKGIKMSKSLKNGIDPQEISNKLGSEIIRLWVAASD
YSGDIAGDDKILARVVDAYRRIRNTLRFLLANTSDFDIEKDGVPLDQLFEIDRYALSRAA
QFQAEILAHYKVYEFHPVVAKLQIYCSEDLGGFYLDILKDRLYTTAPGSLARRSAQTALW
HISQAMLRWMAPFLSFTAEEAWKFVGTGKPGESIFTQTYSKFDAPDEALLAKWNRIREIR
DVVNKDIEAVRAEGKVGSSLQANLQLTAPADDFALLGSLGEDLKFVFITSAIALAAGEAL
STVVTPSAAQKCERCWHYRDDVGHDAAHPTICGRCTSNLFGAGESRSFA