Protein Info for GFF595 in Pseudomonas sp. DMC3

Annotation: Threonylcarbamoyl-AMP synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 185 PF01300: Sua5_yciO_yrdC" amino acids 14 to 181 (168 residues), 160.5 bits, see alignment E=1.6e-51

Best Hits

Swiss-Prot: 95% identical to TSAC_PSEPF: Threonylcarbamoyl-AMP synthase (tsaC) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K07566, putative translation factor (inferred from 95% identity to pfo:Pfl01_0020)

Predicted SEED Role

"TsaC protein (YrdC domain) required for threonylcarbamoyladenosine t(6)A37 modification in tRNA"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (185 amino acids)

>GFF595 Threonylcarbamoyl-AMP synthase (Pseudomonas sp. DMC3)
MVNSWRVQQAAREVRAGAVIAYPTEAVWGLGCDPWNEDAVDRLLAIKNRSVSKGLILVAD
NIRQFDFLFDDFPQDWIDRMASTWPGPNTWLVPHQNLLPEWVTGVHDTVALRVSDHPQVR
DLCSLVGPLISTSANPQGRPPARTRLRIEQYFRGQVDLVLGGSLGGRKNPSVIRDLATGS
VVRPD