Protein Info for GFF5946 in Variovorax sp. SCN45

Annotation: T6SS Serine/threonine protein kinase (EC 2.7.11.1) PpkA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 642 transmembrane" amino acids 400 to 420 (21 residues), see Phobius details amino acids 518 to 538 (21 residues), see Phobius details PF00069: Pkinase" amino acids 59 to 281 (223 residues), 96.4 bits, see alignment E=3e-31 PF07714: PK_Tyr_Ser-Thr" amino acids 60 to 325 (266 residues), 69.1 bits, see alignment E=5.9e-23 PF14326: DUF4384" amino acids 503 to 578 (76 residues), 50.8 bits, see alignment E=2.1e-17

Best Hits

KEGG orthology group: None (inferred from 80% identity to vpe:Varpa_0556)

Predicted SEED Role

"serine/threonine protein kinase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (642 amino acids)

>GFF5946 T6SS Serine/threonine protein kinase (EC 2.7.11.1) PpkA (Variovorax sp. SCN45)
MTDSNDDRTRVVPKPGQPPTTTGDMAATVITAGATPVTSPATGSTDAGLLPVGSRLAEFE
ITRVIGQGGFGVVYEAWDHTLERVVAIKEYLPTSLSTRQQDGTVVPLSERHRETFDLGMR
SFINEARLLAQFDHPSLLKVYRFWQEKGTTYMVMPFYKGDTLREALVAIPAGVDEAWLIR
IMDGVTQALAVMHNANCYHRDIAPDNIILLEGSGRPVVLDFGAARRVITDKTQAITVILK
PGYAPIEQYAEMPDMSQGAWTDVYALAAVMHVAVCGRAPPPSVARLLSDSYVPLAGNDIL
RQRYSPRLLEAIDAGLGVRPEQRPQSMAELRAALDLDAGGHTIAPVPRTQPPRVQQGGGN
GADAATVIVGSAKSTGKTTGNAKAPASPAPAAASGGGKTVVAIASVVALAAVAGGGWWFL
QGRTGGTQADMNNNNEKQQVAVAPTPAPLPDTKVAEAPPPPAPPPPPPPPAPRTPLDSLQ
SLAAGAAPGFDVTATPKKAEVTVGKDRLAFEVRSKREGFVYVFLLSSGGEMFLLFPNLLD
KYNKITAGGSLSLPRASWPMDAGGPPGTDQFAVLVSEHERDFSAAGVQNDGVFPVFPLPV
LAALEATRGSGPSPLLGKPVCAPAGAPCNDVYGVANFKIVEK