Protein Info for GFF592 in Variovorax sp. SCN45

Annotation: Glutamyl-Q tRNA(Asp) synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 TIGR03838: glutamyl-queuosine tRNA(Asp) synthetase" amino acids 4 to 279 (276 residues), 361.6 bits, see alignment E=1.3e-112 PF00749: tRNA-synt_1c" amino acids 6 to 255 (250 residues), 124.2 bits, see alignment E=2.8e-40

Best Hits

Swiss-Prot: 65% identical to GLUQ_LEPCP: Glutamyl-Q tRNA(Asp) synthetase (gluQ) from Leptothrix cholodnii (strain ATCC 51168 / LMG 8142 / SP-6)

KEGG orthology group: K01894, glutamyl-Q tRNA(Asp) synthetase [EC: 6.1.1.-] (inferred from 88% identity to vpe:Varpa_3549)

Predicted SEED Role

"glutamyl-Q-tRNA synthetase" in subsystem Queuosine-Archaeosine Biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.-

Use Curated BLAST to search for 6.1.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (300 amino acids)

>GFF592 Glutamyl-Q tRNA(Asp) synthetase (Variovorax sp. SCN45)
MRETGRFAPSPTGPLHAGSLVAALASWLDVRARGPQARWLVRIEDADTERCLPGMGERIL
RQLADCALLPDEPPVWQTQRTAHYEAALARLKENGLAYPCGCSRKDIDEALARLGLRHER
HGERVYPGTCRDGLHGKPARAWRFAAEKFAGGEICFTDRRLGRQCQDVGREVGDFVLRRA
DGPWAYQLAVVVDDAEQGVTDVVRGEDLTDNTARQILLQRALGFPAPSYLHTPLVRGADG
DKLSKQNGATAIDTSTPEAALAALGAAARALGLRPATATDCAAALAGWVPEWRALYNSRP